Prepare siRNA and miRNA Probes in Just 1 Hour
|mirVana™ miRNA Probe Construction Kit |
With the mirVana miRNA Probe Construction Kit, short (<100 nt) radiolabeled or nonisotopically labeled RNA probes can be generated in less than 1 hour. These probes are ideal for the investigation of a variety of small RNAs. Radiolabeled probes prepared with the mirVana miRNA Probe Construction Kit have been successfully used for the detection of small nuclear RNA (snRNA), small interfering RNA (siRNA), micro RNA (miRNA), and mRNA by RPAs, Northerns and in situ hybridization. Nonisotopically labeled probes generated with the kit have also been used to study the distribution of miRNA or mRNA in tissues by in situ hybridization (See Analysis of miRNA Distribution in Mouse Brain, right).
RNA Probe Synthesis Made Fast, Simple and Inexpensive
Complete Kit for Probe Preparation
Figure 1. miR-16 and miR-22 Expression in Mouse Brain. In situ hybridization was performed on coronal mouse forebrain sections. In situ hybridized cells in the mouse brain cortex (A,B,D,E; 20X magnification) and the head of the caudate nucleus (C,F; 40X magnification) are indicated by arrows. Nonisotopically labeled probes were prepared with Ambion's MAXIscript™ Kit (1.5 kb probe) or with the mirVana miRNA Probe Construction Kit (32 nt long probes, 22 nt target specific sequence).