Multiplex Dynamic Temperature Verification (MDTV) Service
More Confidence. Less Time. Less Money.

Frequent verification of your thermal cyclers will help assess the accuracy and uniformity of the PCR block to ensure that you obtain the optimal performance level from your PCR instrument and have the necessary performance documentation for accreditation. Proper care of your PCR system, which includes frequent temperature verification, will save you from unexpected PCR instrument downtime, minimize downstream sequencing costs, and preserve valuable samples that could be lost due to inaccurate readings.

Save Time and Money While Gaining More Confidence in your Results and Accreditation Compliance with our On-Site MDTV Service

Our on-site Multiplex Dynamic Temperature Verification (MDTV) Service uses an innovative dynamic temperature verification system to measure up to 5 thermal cyclers simultaneously in less than 1 hour. The measurement system, specifically developed for Life Technologies PCR instruments, consists of a 9-channel probe and automated data acquisition and analysis software to deliver the fastest and most accurate temperature readings on your PCR unit.

Only the MDTV Service from Life Technologies can help you:

• Gain greater confidence in your results and accreditation compliance, with more accurate temperature verification assessment and improved documentation
• Save days of PCR system downtime, with an up-to-15-fold faster temperature verification system that has a run time from start to printed results of less than 1 hour.
• Save thousands of dollars in lost sample, wasted reagent, and downstream sequencing costs due to invalid results from inaccurate temperature performance

More Confidence

The MDTV Service provides more accurate readings and improved documentation from highly qualified and certified Life Technologies Field Service Engineers (FSEs) to ensure that you have the highest level of confidence that your PCR system temperature verification is correct and well documented.

• Increased Accuracy—the MDTV Service provides a comprehensive measurement of parameters and increased data sampling resolution of 3 samples per second for 5 accuracy set points:
• temperature accuracy
• temperature uniformity
• temperature stability
• overshoot and undershoot
• ramp rate (up and down)

The MDTV measurement unit also has increased accuracy set points for critical PCR reaction temperatures (60⁰C, 72⁰C, and 95⁰C), as well as the flexibility to change set points for your validated protocols. This helps to ensure that you are consistent with your regulatory requirements for PCR certification, eliminating the need to reoptimize your protocols. The MDTV Service is more accurate than other temperature verification services or in-house tests because temperature verification is performed with the heated cover closed, just like a real cycle run, eliminating environmental air flow distorting the thermal field.

• Improved Documentation—to help meet your accreditation requirements and for improved compliance management, the MDTV Service provides more detailed documentation than is available through other temperature verification services or when performed in-house. Figure 1 shows a passing dynamic stability plot test; the 9 channels measure consistently throughout the run at the 60.0 Degree Celsius set point. Figure 2 shows a failing dynamic stability plot; the 9 channels are consistently grouped across the y access, but the trend varies from -5 Degrees to +5 Degrees above the 85 Degree Celsius set point.

With each MDTV Service, an automated analysis and professional Certificate of Analysis report is provided. Each report contains dynamic plots for each run referencing the original instrument specifications and tracing the national and international standards that will both satisfy regulatory standards for PCR certification and meet internal quality control requirements. Multiple preformatted report options are also available that provide the flexibility of printing either:

Less Time

Unlike most methods that employ static temperature collection with a single probe, the MDTV Service uses a 9-channel unit and rapid data collection system that enables up to 15-fold faster temperature verification. With the multiplex measurement unit used in the MDTV Service, it takes less than 1 hour, from run time to printed results, to perform temperature verification on up to 5 PCR systems simultaneously. This parallel processing allows you to move from days of downtime to only minutes, providing you increased PCR instrument availability.

Less Money

A temperature verification service provides assurance that your PCR unit is running optimally before you run your samples. This can save you thousands of dollars in additional reagent and sample costs that would be incurred to repeat a run or multiple runs on your system. The MDTV Service provides the most accurate temperature verification information for your Life Technologies PCR instrument, so you can trust that your PCR results are correct, minimizing downstream sequencing costs and preserving valuable sample.

With regularly scheduled MDTV Service on-site verifications, operating expenses can be planned and budgeted more effectively. You can save up to 50% on existing temperature verification services and DIY service options when you eliminate the significant expense related to maintaining a calibrated temperature verification tool and perform temperature verifications on 5 or more units in a single Field Service visit.

Flexible MDTV Service Options

The MDTV Service can be requested as a stand-alone service, added to a Life Technologies service contract, or provided along with a planned maintenance service. To add the MDTV Service to an existing service contract or planned maintenance request, reference Cat. No. ZGVM.

Stand Alone Service

For customers with less than 5 thermal cyclers, we will continue to support your temperature verification needs with our standard On-Site Temperature Verification Service.