SelectScreen® Kinase Profiling Services
 Listen to the SelectScreen® team describe how they deliver reliable profiling data on rapid timelines. | Expect and receive high quality kinase profiling data in days and accelerate your lead discovery projects with SelectScreen® Kinase Profiling Services from Life Technologies. Drug discovery labs from pharmaceutical, biotech, and academic institutions have made SelectScreen® the most utilized kinase profiling service in the world1. More than 10,000 projects have been completed through the SelectScreen® Kinase Profiling Service since it’s initiation in 2004. |
Why do scientists trust the SelectScreen® Services team?
- Average data delivery time of just five days - so you can get data to your medicinal chemists more quickly
- All kinase proteins manufactured in-house by Life Technologies – allowing you to utilize the same proteins in your follow-up studies that our team uses in the service
- Broad panel of more than 300 purified protein kinases – to help you establish how selective a compound is to its intended targets
- State-of-the-art compound management system ensures consistently high quality data is delivered for every project
- SelectScreen® labs based in the US and the UK provide profiling services for customers worldwide
- Complete Lab Information Management System (LIMS) ensures project traceability and consistency
Project Options
Customize your kinase profiling project to get the right data for your research.
Standard service options include:
- Targets – 319 available biochemical assays
- Mode – Single point concentrations and IC50 determinations
- Choice of ATP concentration – 10µM, 100µM, and Km [app] ATP (activity assays only)
For the kinases that are available in our activity assays, comprehensive titrations are performed against the Life Technologies panel of available substrates. In addition, ATP titrations are performed for each of these kinases to determine the Km[app] ATP for each target. As a result, you can choose to have your compound run at, above, or below the Km[app] ATP for each kinase within the panel that utilizes an activity assay. This flexibility allows you to identify specific types of inhibitors with various mechanisms of action.
To discuss your kinase profiling project requirements, send us an inquiry or email us.
Technology Overview
Currently, two different assay platforms are available for profiling – activity and binding. The Z’-LYTE® and Adapta® kinase activity assays are used most extensively for profiling and a smaller subset of kinases may be profiled using our LanthaScreen® Eu Kinase Binding Assays.
Overview of available formats
| Z'LYTE® | Technology Overview | Protocol and Assay Conditions |
| Adapta® | Technology Overview | Protocol and Assay Conditions |
| LanthaScreen® Eu Kinase Binding Assay | Technology Overview | Protocol and Assay Conditions |
A stringent validation process ensures the highest quality data possible with each data point performed in duplicate. Strict quality control protocols ensure that any assay results not meeting set specifications will be automatically repeated, this includes a control inhibitor titration (IC50) for each kinase on every plate.
To discuss your kinase profiling project requirements, send us an inquiry or email us.
Broad Assay Panel
| Kinase | Activity Assays | Binding Assay | |
|---|---|---|---|
| Z'-LYTE® | Adapta® | LanthaScreen® | |
| ABL1 |
| ||
| ABL1 E255K |
| ||
| ABL1 G250E |
| ||
| ABL1 T315I |
| ||
| ABL1 Y253F |
| ||
| ABL2 (Arg) |
| ||
| ACVR1 (ALK2) |
| ||
| ACVR1B (ALK4) |
| ||
| ACVR2B |
| ||
| ADRBK1 (GRK2) |
| ||
| ADRBK2 (GRK3) |
| ||
| AKT1 (PKB alpha) |
| ||
| AKT2 (PKB beta) |
| ||
| AKT3 (PKB gamma) |
| ||
| AMPK A1/B1/G1 |
| ||
| AMPK A2/B1/G1 |
| ||
| AURKA (Aurora A) |
| ||
| AURKB (Aurora B) |
| ||
| AURKC (Aurora C) |
| ||
| AXL |
| ||
| BLK |
| ||
| BMPR1A (ALK3) |
| ||
| BMX |
| ||
| BRAF |
|
| |
| BRAF V599E |
|
| |
| BRSK1 (SAD1) |
| ||
| BTK |
| ||
| CAMK1 (CaMK1) |
| ||
| CAMK1D (CaMKI delta) |
| ||
| CAMK2A (CaMKII alpha) |
| ||
| CAMK2B (CaMKII beta) |
| ||
| CAMK2D (CaMKII delta) |
| ||
| CAMK4 (CaMKIV) |
| ||
| CAMKK1 (CAMKKA) |
| ||
| CAMKK2 (CaMKK beta) |
| ||
| CDC42 BPA (MRCKA) |
| ||
| CDC42 BPB (MRCKB) |
| ||
| CDK1/cyclin B |
| ||
| CDK2/cyclin A |
| ||
| CDK5/p25 |
| ||
| CDK5/p35 |
| ||
| CDK7/cyclin H/MNAT1 |
| ||
| CDK8/cyclin C |
| ||
| CDK9/cyclin K |
| ||
| CDK9/cyclin T1 |
| ||
| CHEK1 (CHK1) |
| ||
| CHEK2 (CHK2) |
| ||
| CHUK (IKK alpha) |
| ||
| CLK1 |
| ||
| CLK2 |
| ||
| CLK3 |
| ||
| CLK4 |
| ||
| CSF1R (FMS) |
| ||
| CSK |
| ||
| CSNK1A1 (CK1 alpha 1) |
| ||
| CSNK1D (CK1 delta) |
| ||
| CSNK1E (CK1 epsilon) |
| ||
| CSNK1G1 (CK1 gamma 1) |
| ||
| CSNK1G2 (CK1 gamma 2) |
| ||
| CSNK1G3 (CK1 gamma 3) |
| ||
| CSNK2A1 (CK2 alpha 1) |
| ||
| CSNK2A2 (CK2 alpha 2) |
| ||
| DAPK1 |
| ||
| DAPK3 (ZIPK) |
| ||
| DCAMKL2 (DCK2) |
| ||
| DDR1 |
| ||
| DDR2 |
| ||
| DMPK |
| ||
| DNA-PK |
| ||
| DYRK1A |
| ||
| DYRK1B |
| ||
| DYRK3 |
| ||
| DYRK4 |
| ||
| EEF2K |
| ||
| EGFR (ErbB1) |
| ||
| EGFR (ErbB1) L858R |
| ||
| EGFR (ErbB1) L861Q |
| ||
| EGFR (ErbB1) T790M |
| ||
| EGFR (ErbB1) T790M L858R |
| ||
| EPHA1 |
| ||
| EPHA2 |
| ||
| EPHA3 |
| ||
| EPHA4 |
| ||
| EPHA5 |
| ||
| EPHA7 |
| ||
| EPHA8 |
| ||
| EPHB1 |
| ||
| EPHB2 |
| ||
| EPHB3 |
| ||
| EPHB4 |
| ||
| ERBB2 (HER2) |
| ||
| ERBB4 (HER4) |
| ||
| FER |
| ||
| FES (FPS) |
| ||
| FGFR1 |
| ||
| FGFR2 |
| ||
| FGFR3 |
| ||
| FGFR3 K650E |
| ||
| FGFR4 |
| ||
| FGR |
| ||
| FLT1 (VEGFR1) |
| ||
| FLT3 |
| ||
| FLT3 D835Y |
| ||
| FRAP1 (mTOR) |
| ||
| FRK (PTK5) |
| ||
| FYN |
| ||
| GRK4 |
| ||
| GRK5 |
| ||
| GRK6 |
| ||
| GRK7 |
| ||
| GSG2 (Haspin) |
| ||
| GSK3A (GSK3 alpha) |
| ||
| GSK3B (GSK3 beta) |
| ||
| HCK |
|
| |
| HIPK1 (Myak) |
| ||
| HIPK2 |
| ||
| HIPK3 (YAK1) |
| ||
| HIPK4 |
| ||
| IGF1R |
| ||
| IKBKB (IKK beta) |
| ||
| IKBKE (IKK epsilon) |
| ||
| INSR |
| ||
| INSRR (IRR) |
| ||
| IRAK1 |
| ||
| IRAK4 |
| ||
| ITK |
| ||
| JAK1 |
| ||
| JAK2 |
| ||
| JAK2 JH1 JH2 |
| ||
| JAK2 JH1 JH2 V617F |
| ||
| JAK3 |
| ||
| KDR (VEGFR2) |
| ||
| KIT |
| ||
| KIT T670I |
| ||
| KIT V654A |
| ||
| LIMK1 |
| ||
| LIMK2 |
| ||
| LRRK2 |
| ||
| LRRK2 G2019S |
| ||
| LTK (TYK1) |
| ||
| LYN A |
| ||
| LYN B |
| ||
| MAP2K1 (MEK1 ) |
|
| |
| MAP2K1 (MEK1) S218D S222D |
| ||
| MAP2K2 (MEK2) |
|
| |
| MAP2K3 (MEK3) |
| ||
| MAP2K6 (MKK6) |
|
| |
| MAP2K6 (MKK6) S207E T211E |
| ||
| MAP3K10 (MLK2) |
| ||
| MAP3K11 (MLK3) |
| ||
| MAP3K14 (NIK) |
| ||
| MAP3K2 (MEKK2) |
| ||
| MAP3K3 (MEKK3) |
| ||
| MAP3K5 (ASK1) |
| ||
| MAP3K7/MAP3K7IP1 (TAK1-TAB1) |
| ||
| MAP3K8 (COT) |
| ||
| MAP3K9 (MLK1) |
| ||
| MAP4K2 (GCK) |
| ||
| MAP4K4 (HGK) |
| ||
| MAP4K5 (KHS1) |
| ||
| MAPK1 (ERK2) |
| ||
| MAPK10 (JNK3) |
|
| |
| MAPK11 (p38 beta) |
| ||
| MAPK12 (p38 gamma) |
| ||
| MAPK13 (p38 delta) |
| ||
| MAPK14 (p38 alpha) |
| ||
| MAPK14 (p38 alpha) Direct |
| ||
| MAPK3 (ERK1) |
| ||
| MAPK8 (JNK1 ) |
|
| |
| MAPK9 (JNK2) |
|
| |
| MAPKAPK2 |
| ||
| MAPKAPK3 |
| ||
| MAPKAPK5 (PRAK) |
| ||
| MARK1 (MARK) |
| ||
| MARK2 |
| ||
| MARK3 |
| ||
| MARK4 |
| ||
| MATK (HYL) |
| ||
| MELK |
| ||
| MERTK (cMER) |
| ||
| MET (cMet) |
| ||
| MET M1250T |
| ||
| MINK1 |
| ||
| MKNK1 (MNK1) |
| ||
| MKNK2 (MNK2) |
| ||
| MLCK (MLCK2) |
| ||
| MST1R (RON) |
| ||
| MST4 |
| ||
| MUSK |
| ||
| MYLK (MLCK) |
| ||
| MYLK2 (skMLCK) |
| ||
| NEK1 |
| ||
| NEK2 |
| ||
| NEK4 |
| ||
| NEK6 |
| ||
| NEK7 |
| ||
| NEK9 |
| ||
| NLK |
| ||
| NTRK1 (TRKA) |
| ||
| NTRK2 (TRKB) |
| ||
| NTRK3 (TRKC) |
| ||
| NUAK1 (ARK5) |
| ||
| PAK1 |
| ||
| PAK2 (PAK65) |
| ||
| PAK3 |
| ||
| PAK4 |
| ||
| PAK6 |
| ||
| PAK7 (KIAA1264) |
| ||
| PASK |
| ||
| PDGFRA (PDGFR alpha) |
| ||
| PDGFRA D842V |
| ||
| PDGFRA T674I |
| ||
| PDGFRA V561D |
| ||
| PDGFRB (PDGFR beta) |
| ||
| PDK1 |
| ||
| PDK1 Direct |
| ||
| PHKG1 |
| ||
| PHKG2 |
| ||
| PI4KA |
| ||
| PI4KB (PI4K beta) |
| ||
| PIK3C2A (PI3K-C2 alpha) |
| ||
| PIK3C2B (PI3K-C2 beta) |
| ||
| PIK3C3 (hVPS34) |
| ||
| PIK3CA/PIK3R1 (p110 alpha/p85 alpha) |
| ||
| PIK3CD/PIK3R1 (p110 delta/p85 alpha) |
| ||
| PIM1 |
| ||
| PIM2 |
| ||
| PKN1 (PRK1) |
| ||
| PLK1 |
| ||
| PLK2 |
| ||
| PLK3 |
| ||
| PRKACA (PKA) |
| ||
| PRKCA (PKC alpha) |
| ||
| PRKCB1 (PKC beta I) |
| ||
| PRKCB2 (PKC beta II) |
| ||
| PRKCD (PKC delta) |
| ||
| PRKCE (PKC epsilon) |
| ||
| PRKCG (PKC gamma) |
| ||
| PRKCH (PKC eta) |
| ||
| PRKCI (PKC iota) |
| ||
| PRKCN (PKD3) |
| ||
| PRKCQ (PKC theta) |
| ||
| PRKCZ (PKC zeta) |
| ||
| PRKD1 (PKC mu) |
| ||
| PRKD2 (PKD2) |
| ||
| PRKG1 |
| ||
| PRKG2 (PKG2) |
| ||
| PRKX |
| ||
| PTK2 (FAK) |
| ||
| PTK2B (FAK2) |
| ||
| PTK6 (Brk) |
| ||
| RAF1 (cRAF) Y340D Y341D |
|
| |
| RET |
| ||
| RET V804L |
| ||
| RET Y791F |
| ||
| RIPK2 |
| ||
| ROCK1 |
| ||
| ROCK2 |
| ||
| ROS1 |
| ||
| RPS6KA1 (RSK1) |
| ||
| RPS6KA2 (RSK3) |
| ||
| RPS6KA3 (RSK2) |
| ||
| RPS6KA4 (MSK2) |
| ||
| RPS6KA5 (MSK1) |
| ||
| RPS6KA6 (RSK4) |
| ||
| RPS6KB1 (p70S6K) |
| ||
| SGK (SGK1) |
| ||
| SGK2 |
| ||
| SGKL (SGK3) |
| ||
| SLK |
| ||
| SNF1LK2 |
| ||
| SRC |
| ||
| SRC N1 |
| ||
| SRMS (Srm) |
| ||
| SRPK1 |
| ||
| SRPK2 |
| ||
| STK16 (PKL12) |
| ||
| STK17A (DRAK1) |
| ||
| STK22B (TSSK2) |
| ||
| STK22D (TSSK1) |
| ||
| STK23 (MSSK1) |
| ||
| STK24 (MST3) |
| ||
| STK25 (YSK1) |
| ||
| STK3 (MST2 ) |
| ||
| STK33 |
| ||
| STK4 (MST1) |
| ||
| SYK |
| ||
| TAOK2 (TAO1) |
| ||
| TAOK3 (JIK) |
| ||
| TBK1 |
| ||
| TEC |
| ||
| TEK (Tie2) |
| ||
| TGFBR1 (ALK5) |
| ||
| TNK2 (ACK) |
| ||
| TTK |
| ||
| TXK |
| ||
| TYK2 |
| ||
| TYRO3 (RSE) |
| ||
| WEE1 |
| ||
| WNK2 |
| ||
| YES1 |
| ||
| ZAK |
| ||
| ZAP70 |
| ||
Kinase Biology Tools
With the broadest collections of assays, high-throughput screening and profiling services, and access to customized assays and tools designed to meet your specifications, your lab can choose the right kinase biology tools and services for your exact stage of research.
Personalized Support
 | From project beginning to end, you can rely on our Drug Discovery Services project management team to provide frequent project communication via a single point of contact. Watch this two-minute video to see how our project managers and technical specialists create an exceptional experience for our customers. If you have already received a quote number, place an order here. |
Footnotes:
1 – Source: HTStec report, 2011
For Research Use Only. Not for use in diagnostic procedures.
