SelectScreen® Cell-based Pathway Profiling Services

Strength in Numbers - Drug Discovery Services
Listen to the SelectScreen® team describe
how they deliver reliable profiling data on rapid timelines.
    

Expect and receive high quality cellular profiling data in days and accelerate your lead discovery projects with SelectScreen® Cell-based Pathway Profiling Services from Life Technologies

Drug discovery labs from pharmaceutical, biotech, and academic institutions have utilized SelectScreen® Cell-based Pathway Profiling Service since their initiation in 2006.  To date, our SelectScreen® team has completed more than 500 cell-based pathway profiling projects – with an on-time delivery rate higher than 99%.

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Why do scientists trust the SelectScreen® Services team?

  • Average data delivery time of just 7 days - so you can get data to your medicinal chemists more quickly
  • All assays and reagents manufactured in-house by Life Technologies – allowing you to utilize the same cellular assays in your follow-up studies that our team uses in the service
  • Broad panel of more than 118 cellular assays available
  • State-of-the-art compound management system ensures consistently high quality data is delivered for every project
  • Complete Lab Information Management System (LIMS) ensures project traceability and consistency

Customize your cell-based profiling project to get the right data for your research.

Standard service options include:

  • Targets – 38 Pathways (72 assays)  and 13 LanthaScreen® cell lines (46 assays) available
  • Mode – IC50 and/or EC50 determinations

Biochemical kinase profiling services are also available for clients seeking both biochemical and cellular data.

To discuss your cell-based profiling project requirements, send us an inquiry or email us.

Currently, two different assay platforms are available for profiling - CellSensor® reporter gene assays and LanthaScreen® cellular TR-FRET assays.

Overview of available formats

CellSensor® cell linesTechnology OverviewProtocol and Assay Conditions
LanthaScreen® cellular assaysTechnology OverviewProtocol and Assay Conditions

A stringent validation process ensures the highest quality data possible with each data point performed in duplicate.  Strict quality control protocols ensure that any assay results not meeting set specifications will be automatically repeated, this includes a control inhibitor titration for each cell-based assay on every plate.

To discuss your cell-based profiling project requirements, send us an inquiry or email us. 

If your target of interest is not on the list below, additional CellSensor® Cell Lines may be profiled using our Custom Services. View the entire list of CellSensor® Cell Lines available.

Pathway Cell Line Activator Inhibitor Antibody CellSensor® Cell Lines LanthaScreen Cellular Assay
Anti-oxidant Response ARE-bla HepG2 tBHQ Ro-31-8220 N/A

 
B cell receptor NFAT-bla RA-1 Goat anti-Human IgM Syk Inhibitor II

N/A

 
cAMP/PKA CRE-bla HEK 293T Forskolin H-89

N/A

 
cAMP/PKA CRE-bla Jurkat Forskolin H-89

N/A

 
DNA damage/p53 Response p53RE-bla HCT-116 Mitomycin N/A

N/A

 
ER Stress Response ESRE-bla HeLa Tunicamycin N/A

N/A

 
Glucocorticoid Receptor MMTV-bla HeLa Dexamethasone RU-486

N/A

 
Heat Shock Response HSE-bla HeLa 17-AAG Quercetin

N/A

 
Hypoxia HRE-bla ME-120 CoCl2 Chetomin

N/A

 
Interleukin 4/STAT6 STAT6-bla RA1 IL-4 JAK Inhibitor I

N/A

 
JAK/STAT irf1-bla HEL N/A JAK Inhibitor I

N/A

 
JAK/STAT ISRE-bla HEK 293T IFN-alpha JAK Inhibitor I

N/A

 
JAK/STAT ISRE-bla Jurkat IFN-alpha JAK Inhibitor I

N/A

 
JAK/STAT SIE-bla HEK 293T IFN-gamma JAK Inhibitor I

N/A

 
JAK/STAT SIE-bla HEK 293T IL-6 JAK Inhibitor I

N/A

 
JAK/STAT SIE-bla ME-120 IL-6 JAK Inhibitor I

N/A

 
JAK/STAT SIE-bla ME-120 IFN-gamma JAK Inhibitor I

N/A

 
JAK2/STAT5 irf1-bla TF1 GM-CSF JAK Inhibitor I

N/A

 
JAK2/STAT5 irf1-bla TF1 EPO JAK Inhibitor I

N/A

 
MAPK c-fos-bla HEK 293T PMA U0126

N/A

 
MAPK c-fos-bla ME-120

EGF		 PD153035

N/A

 
MAPK/EGFR/Ras/Raf AP1-bla ME-120 EGF PD153035

N/A

 
MAPK/MEK/B-raf AP1-bla A375 N/A Raf1 Kinase Inhibitor

N/A

 
NFkB NFkB-bla Jurkat TNF-alpha Withaferin A

N/A

 
NFkB NFkB-bla ME-120 TNF-alpha Withaferin A

N/A

 
NFkB NFkB-bla ME-120 IL-1 beta Withaferin A

N/A

 
NFkB NFkB-bla THP-1 TNF-alpha Withaferin A

N/A

 
PI3K/AKT/FOXO3 TREx FOXO3-DBE-bla HeLa Insulin Triciribine

N/A

 
PKC & Ca2+ TrkA-NFAT-bla CHO-K1 NGF-2.5S K252a

N/A

 
PKC & Ca2+ TrkB-NFAT-bla CHO-K1 BDNF K252a

N/A

 
PKC & Ca2+ TrkC-NFAT-bla CHO-K1 Neurotrophin-3

K252a

N/A

 
PKC/Ca2+ NFAT-bla Jurkat Thapsigargin

Ro-31-8220

N/A

 
T Cell Receptor NFAT-bla Jurkat anti-CD3:CD28 Ro-31-8220

N/A

 
TGF-beta SBE-bla HEK 293T TGF-beta TGF-beta R1 Kinase Inhibitor

N/A

 
TNF-alpha/JNK AP1-bla ME-120 TNF-alpha JNK Inhibitor II

N/A

 
Toll-Like Receptor (TLR4) NFkB-bla THP-1 LPS Withaferin A

N/A

 
Wnt/Beta-Catenin LEF/TCF-bla HCT-116 Wnt-3A ICG-001

N/A

 
Wnt/Beta-Catenin (APC-/-) LEF/TCF-bla SW 420 None ICG-001

N/A

 
NFkB IkB GripTite™ (293) TNF-alpha BMS345541 Tb-anti-plkB[pSer32]  

MAPK c-Jun (1-79) HeLa TNF-alpha or Anisomycin JNK Inhibitor II Tb-anti-c-Jun[pSer73]  

MAPK ATF2 (19-106) A549 TNF-alpha or Anisomycin JNK Inhibitor II Tb-anti-AFT2[pThr71]  

MAPK* ERK2 U2OS EGF or PDGF U0126 Tb-anti-ERK2[pThr185/Tyr187]  

MAPK ERK2 A375 (B-RAF V600E) None Raf1 Kinase Inhibitor Tb-anti-ERK2[pThr185/pTyr187]  

PI3K/AKT PRAS40 HEK 293E Insulin or IGF-1 PI-103 Tb-anti-PRAS40[pThr246]  

PI3K/AKT PRAS40 HEK 293E Insulin or IGF-1 PI-103 Tb-anti-PRAS40[pSer183]  

PI3K/AKT PDCD4 HEK 293E Insulin or IGF-1 Rapamycin Tb-anti-PDCD4[pSer457]  

PI3K/AKT AKT HEK 293E IGF-1 PI-103 Tb-anti-AKT[pSer473]  

PI3K/AKT AKT HEK 293E IGF-1 PI-103

Tb-anti-AKT (pThr308)		  

PI3K/AKT IGF-1R GripTite™ (293) IGF-1 PQ401

Tb-PY20		  

JAK/STAT STAT1 U2OS IFN-gamma or IFN-alpha JAK Inhibitor I Tb-STAT1[pTyr701]  

JAK/STAT STAT3 GripTite™ IL-6 or IFN-alpha JAK Inhibitor I Tb-STAT3[pTyr705]  

JAK/STAT STAT5 TF-1 GM-CSF or EPO JAK Inhibitor I Anti-STAT5 A/B[pTyr694/699]  

     JAK/STAT STAT5 U2OS (JAK2 V617F) None JAK Inhibitor I Anti-STAT5 A/B[pTyr694/699]  

Choose the right reagents, assays and services for your cell-based pathway profiling project. In addition to our SelectScreen® Cell-Based Pathway Profiling Services, we offer a broad selection of tools for “do it yourself” projects.

Personalized Support

From project beginning to end, you can rely on our Drug Discovery Services project management team to provide frequent project communication via a single point of contact.

Watch this two-minute video to see how our project managers and technical specialists create an exceptional experience for our customers.

If you have already received a quote number, place an order here.

For Research Use Only. Not for use in diagnostic procedures.

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