Proven Plasmid Purity
|Plasmid DNA purified by the Plasmid DNA Purification Service undergoes rigorous, comprehensive quality control (QC) testing (Table 1) to meet the high standards set in your service agreement. A complete Certificate of Analysis† is provided with each lot. |
† Plasmid DNA prepared from the 1 L and BAC 1 L and 5 L prep cultures is provided with a certificate of conformance.
Table 1. Stringent QC specifications and passing results for the Plasmid DNA Purification Service.
|Visual appearance||Clear, colorless solution; no foreign particles|
|DNA homogeneity||Predominantly closed circular, as determined by agarose gel electrophoresis|
|Identity||Comigrates with reference material or supercoiled plasmid marker, as determined by agarose gel electrophoresis|
|DNA concentration||Concentration determined by absorbance at 260 nm|
|Purity||A260/A280 ratio of 1.8–2.0|
|Residual RNA||Not visible by agarose gel electrophoresis|
|Residual genomic DNA||Less than 5%, as determined by agarose gel electrophoresis at 200 ng load|
|Endotoxin content||<5000 EU/mg (standard service) |
<100 EU/mg (additional fees apply)
<5 EU/mg (additional fees apply)
|Sterility (bioburden test)*||No observed growth on LB plate in 48 hr|
|Residual protein*||Less than 2%, as determined by BCA assay|
|Restriction enzyme digestion*||Digestion analysis of final product using restriction enzyme(s) of choice providing the expected banding pattern|
*Available upon request. Additional charges may apply.