Proven Plasmid Purity

Plasmid DNA purified by the Plasmid DNA Purification Service undergoes rigorous, comprehensive quality control (QC) testing (Table 1) to meet the high standards set in your service agreement. A complete Certificate of Analysis† is provided with each lot.

† Plasmid DNA prepared from the 1 L and BAC 1 L and 5 L prep cultures is provided with a certificate of conformance.

Table 1. Stringent QC specifications and passing results for the Plasmid DNA Purification Service.

Test Results
Visual appearance Clear, colorless solution; no foreign particles
DNA homogeneity Predominantly closed circular, as determined by agarose gel electrophoresis
Identity Comigrates with reference material or supercoiled plasmid marker, as determined by agarose gel electrophoresis
DNA concentration Concentration determined by absorbance at 260 nm
Purity A260/A280 ratio of 1.8–2.0
Residual RNA Not visible by agarose gel electrophoresis
Residual genomic DNA Less than 5%, as determined by agarose gel electrophoresis at 200 ng load
Endotoxin content <5000 EU/mg (standard service)
<100 EU/mg (additional fees apply)
<5 EU/mg (additional fees apply)
Sterility (bioburden test)* No observed growth on LB plate in 48 hr
Residual protein* Less than 2%, as determined by BCA assay
Restriction enzyme digestion* Digestion analysis of final product using restriction enzyme(s) of choice providing the expected banding pattern

*Available upon request. Additional charges may apply.
For more information, call 800.955.6288 x2, or e-mail custom.services@lifetech.com. 
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