Custom TOPO® Adaptation Services
The TOPO® Cloning Advantage
Invitrogen's TOPO® cloning technology simplifies and improves PCR cloning by replacing ligase and long ligation times with a cloning vector that has been “activated” with topoisomerase I. This activated vector supports room-temperature ligation in 5 minutes and yields a very high percentage of recombinants. Simply put, no other cloning technology is as fast or as efficient.
How It Works
Functionally tested TOPO®-adapted custom vectors are supplied in bulk, for a minimum of 500 reactions, so they are HTP compatible. In addition, you'll receive high-efficiency competent cells in bulk or in a 96-well format for HTP cloning. You won't have to gather additional reagents to complete your cloning projects—so you'll save even more time.
Activate YOUR Vector!
- No need to switch protocols and reagents to a standard TOPO® vector—you can continue to use the vector that best suits your cloning needs, yet still enjoy the advantage of 5-minute ligations at room temperature
- Greatly increased cloning efficiency over non-adapted vectors
- No need to change any downstream assays or experimental parameters