Flp-In™ Cell Line Development

Single-Copy Isogenic Cell Lines

Some stable cell lines may contain multiple integrations of a gene of interest. Flp-In™ technology stably delivers one copy of your gene to a single site, delivering isogenic cell lines.

 

Overview

The Flp-In™ system delivers a single, stable integration at a single FRT site at a transcriptionally active genomic locus. Once the FRT site is inserted, expression constructs may be easily inserted into the locus, permitting direct comparisons of different constructs (e.g., gene families, point mutations).

Invitrogen Custom Services can develop either Flp-In™ host cells or expression cells, depending on your needs and budget. Flp-In™ cells can also be adapted to use T-REx™ inducible expression technology.

Development Options

  • Host cell lines— Let our services team establish and validate in your cell line of interest with a single site insertion for the recombination of your expression plasmid.
  • Expression cell lines— Let our services team recombine your expression plasmid and validate expression in your Host FlpIn™ cell line of interest creating an isogenic expression cell line ready for use in your experiments.

Frequently Asked Questions

Q. What are the differences between Flp-In™ and Jump-In™ cell lines?
A. The host Flp-In™ cell line is generated from a random integration, as opposed to integration at a specific genomic location with the Jump-In™ system and there are licensing differences. Please contact Technical Support for more information.

Q. Does Invitrogen have predeveloped Flp-In™ cell lines?
A. Yes, we offer a number of Flp-In™-adapted cell lines, including 293, CHO, and 3T3 among others. Please contact Technical Support for more information.
For more information, call 800.955.6288 x2, or e-mail custom.services@lifetech.com.   A project manager will respond to your inquiry within 2 business days.