- SelectScreen® Kinase Profiling Service
- SelectScreen® Cell-based GPCR Profiling Services
- SelectScreen® Cell-based Pathway Profiling Services
- SelectScreen® Cell-based Nuclear Receptor Profiling Services
- SelectScreen® P450 Profiling Service
- SelectScreen® hERG Screening Service
- SelectScreen® Library Screening Service
- Experience SelectScreen® Services
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SelectScreen® Kinase Profiling Services
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The SelectScreen® Kinase Profiling Service allows you to determine the specificity of your compounds using a panel of human kinases. Profiling against a broad kinase panel establishes how selective a compound is to its intended target/s.
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Choose from a broad panel of kinase assays
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The service utilizes an expanding library of purified protein kinase targets, all of which are manufactured by Invitrogen. Currently, two different assay platforms are available for profiling – activity and binding. The Z’-LYTE® and Adapta® kinase activity assays are used most extensively for profiling and a smaller subset of kinases may be profiled using our LanthaScreen® Eu Kinase Binding Assays.
Overview of available formats
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Design your profiling project
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We can profile your compounds against any number of available targets. Listed in the table below are the kinases available for profiling. Not sure which kinases you would like to include in your SelectScreen® project? We’ve made the selection easier for you through the creation of the following preset panels:
Oncology-related kinase panel
Cell cycle-related kinase panel
Kinome Sampler
Standard service options include:- Targets – 308 available biochemical assays
- Mode – Single point concentrations and IC50 determinations
- Choice of ATP concentration – 10µM, 100µM, and Km [app] ATP (activity assays only)
A stringent validation process ensures the highest quality data possible with each data point performed in duplicate. Strict quality control protocols ensure that any assay results not meeting set specifications will be automatically repeated, this includes a control inhibitor titration (IC50) for each kinase on every plate.
For the kinases that are available in our activity assays, comprehensive titrations are performed against Invitrogen’s panel of available substrates. In addition, ATP titrations are performed for each of these kinases to determine the Km[app] ATP for each target. As a result, you can choose to have your compound run at, above, or below the Km[app] ATP for each kinase within the panel that utilizes an activity assay. This flexibility allows you to identify specific types of inhibitors with various mechanisms of action.
Additional options for profiling may include, but are not limited to:- Kinases not within our standard panel
- Compound preincubation
- Analysis of slow binding inhibitors
- Analysis of compound off rates
- Characterization of type II inhibitors using active and nonactive kinases
Email us at discoveryservices@invitrogen.com for more information about additional options available for your project.
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Request Additional Information
To learn more about our service, screening options, or pricing, click here.
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Ordering Information
The SelectScreen® Kinase Profiling Service is straightforward and efficient to utilize. No subscriptions or enrollment are necessary. Projects will proceed through the following steps:
1. Fill out the SelectScreen® Kinase Profiling Submission Form
2. Receive confirmation from Invitrogen with scope of project and pricing
3. Supply purchase order and prepare compounds for submission to Invitrogen
4. Receive an email report with the results within two weeks
If you have received a quote number and would like to place an order, click here
1. Fill out the SelectScreen® Kinase Profiling Submission Form
2. Receive confirmation from Invitrogen with scope of project and pricing
3. Supply purchase order and prepare compounds for submission to Invitrogen
4. Receive an email report with the results within two weeks
If you have received a quote number and would like to place an order, click here
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