Pre-designed siRNA's - How Much Do I Need?
| Number of Transfections 5 nmol of siRNA | ||
|---|---|---|
| Plate Type (Transfection Vol.) | 10 nM Final siRNA Concentration | 100 nM Final siRNA Concentration |
| 6 well (2.5 ml) | 200 | 20 |
| 12 well (1 ml) | 500 | 50 |
| 24 well (0.5 ml) | 100 | 10 |
| 96 well (0.1 ml) | 5000 | 50 |
| Number of Transfections 20 nmol of siRNA | ||||
|---|---|---|---|---|
| Plate Type (Transfection Vol.) | 10 nM Final siRNA Concentration | 100 nM Final siRNA Concentration | ||
| 6 well (2.5 ml) | 800 | 80 | ||
| 12 well (1 ml) | 2000 | 200 | ||
| 24 well (0.5 ml) | 4000 | 400 | ||
| 96 well (0.1 ml) | 20,000 | 2000 | ||
| Number of Transfections 40 nmol of siRNA | ||||
|---|---|---|---|---|
| Plate Type (Transfection Vol.) | 10 nM Final siRNA Concentration | 100 nM Final siRNA Concentration | ||
| 6 well (2.5 ml) | 1600 | 160 | ||
| 12 well (1 ml) | 4000 | 400 | ||
| 24 well (0.5 ml) | 8000 | 800 | ||
| 96 well (0.1 ml) | 40,000 | 4000 | ||
