Silencer® Select siRNA Libraries, Made to Order
What are Silencer® Select siRNAs?
Silencer Select siRNAs are the next generation Ambion siRNAs. These siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications to yield siRNAs with unrivalled efficacy, potency, and specificity. In siRNA screening applications, the result is fewer false negatives due to poor silencing and fewer false positives due to off-target effects. In other words, with Silencer Select siRNAs you get cleaner, more consistent data from your primary screen, so you expend less time and resources to find true, validated targets. The result is fewer failed experiments due to poor silencing, and cleaner, more consistent phenotypic data. For more information, including data, brochure, webinar, and FAQs, please visit our Silencer Select Information Page.
Rigorous Quality Control for Results You Can Trust
Life Technologies synthesizes all siRNAs in house to exacting quality standards. After synthesis and purification using extensively validated and controlled procedures, each RNA oligonucleotide is assessed by MALDI-TOF (matrix-assisted laser desorption ionization—time-of-flight) mass spectrometry to confirm siRNA identity and quality. After annealing, each siRNA duplex is analyzed by nondenaturing gel electrophoresis to confirm proper annealing. And to follow each siRNA from order to delivery, we use a proprietary, state-of-the-art electronic tracking system. The result is premium quality siRNA you can trust.
Pre-defined siRNA Libraries Plated to Order
Pre-defined, 0.25 nmol Silencer® Select siRNA Libraries are available for human:
- GPCRs (non-olfactory)
- Ion channels
- Nuclear hormone receptors
- Druggable genome
- Extended druggable genome
To create these siRNA libraries, the Life Technologies bioinformatics team uses PANTHER (www.pantherdb.org) and Gene Ontology™ annotation information to create up-to-date target gene sets. siRNAs targeting the collection are then plated to order (minimum 1 nmol), giving you not only the most up-to-date siRNA libraries, but also the freedom to customize your library so that it is most convenient for your particular experimental setup. Want to add siRNAs to your collection? No problem! We can also provide siRNAs in 96 well or 384 well plates, deliver multiple aliquots of each plate, arrange siRNAs in custom configurations, and provide siRNAs individually and/or in pools.
(Our most popular collections are also available as pre-plated, ready-to-ship libraries with 0.25 nmol of each siRNA.)
Custom siRNA Libraries—The Ultimate in Flexibility
Do you have a favorite list of human genes you would like to target with a collection of siRNAs? We can prepare a custom Silencer Select siRNA Library to any set of human genes. The minimum order is only 36 siRNAs. All you need to supply is a list of genes or transcripts identified by NCBI Entrez Gene ID or RefSeq mRNA accession number. With this user-friendly option, you can specify the order of the siRNAs on the plate, as well as the amount of siRNA per order (1 nmol minimum), number of aliquots of each plate, and the number of siRNAs per target. siRNAs can be provided individually or pooled.
Information to Accelerate Discovery
All Silencer Select siRNA Libraries, whether custom, pre-defined, or pre-plated, are supplied with full siRNA sequence information, and when available, with siRNA validation data. Also provided on the CD accompanying the siRNA library are gene annotation information, which includes gene symbol, gene name, aliases, NCBI Entrez Gene ID, associated RefSeq mRNA accession numbers, and other accession numbers. Plates are individually barcoded with unique identifiers; this information is also provided with the siRNA and gene information.