siRNA
 | RNA interference (RNAi) is the best way to effectively knock down gene expression to study protein function in a wide range of cell types. Traditional RNAi methods for gene knockdown in mammalian cells involved the use of synthetic RNA duplexes consisting of two unmodified 21mer oligonucleotides annealed together to form short/small interfering RNAs (siRNAs). Silencer® Select siRNA and Stealth RNAi™ siRNA improve upon these traditional duplexes by using proprietary chemical modifications to ensure better RNAi results. |
Which siRNA is Right for You?
| Cost-effective siRNA | Good knock-down, low off-target | Highest knock-down, lowest off-target | |||
|---|---|---|---|---|---|
| Potency | 100 nM recommended conc. |
20 nM recommended conc. |
5 nM recommended conc. |
||
| Efficacy (>70% knockdown) | 2 of 3 siRNA guaranteed | 2 of 3 siRNA guaranteed | 2 of 2 siRNA guaranteed | ||
| Target Specificity | Moderate | High | Highest | ||
| Innate Immune Response | Minimum | Minimized through chemical modifications | Minimized through chemical modifications | ||
| Molecular Format | Unmodified 21-bp duplex with overhangs | Modified 25-bp duplex with no overhangs | LNA modified 21-bp duplex with overhangs | ||
| Coverage | Coding RNA | Coding RNA | Coding & Non-coding RNA | ||
| Target Species | Human, Mouse, Rat (Other species: use custom tool) |
Human, Mouse, Rat (Other species: use custom tool) |
Human, Mouse, Rat (Other species: use custom tool) |
||
| Custom Design Tool | Design custom Silencer® siRNA | Design custom Stealth Select RNAi™ siRNA | Design custom Silencer® Select siRNA | ||
| Recommended Catalog # |
AM16708: Silencer® Pre-designed siIRNA 5 nmol | 1299003: RNA-Stealth Select Oligos Tube Set | 4392420: Silencer® Select Pre-Designed siRNA 5 nmol | ||
| Order Now | Order Now | Order Now |
siRNA Libraries
|
siRNA Controls
|
Additional siRNA Ordering Tools |
Questions?If you have any questions on products or ordering, please contact us at RNAiresearcher@lifetech.com |
