TaqMan® MicroRNA Assays & Arrays

TaqMan® MicroRNA Assays—Unrivaled sensitivity, specificity, and dynamic range
Our MicroRNA solutions feature TaqMan® MicroRNA Assay sensitivity, specificity, and dynamic range for unsurpassed miRNA quantitation. Our comprehensive Applied Biosystems solution provides the flexibility to meet different experimental needs and is supported by a complete range of reagents, assays, and real-time PCR instruments.

MicroRNA Assay & Array Workflows: Find the Products You Need for Every Step

Get Same-Day Results with Megaplex™ Primer Pools and TaqMan® MicroRNA Arrays

  • Megaplex™ Primer Pools simplify and accelerate miRNA profiling by reducing hands-on time and the amount of sample you need to as little as 1ng of total human, mouse or rat RNA—up to 100 times less material than existing analysis tools.
  • Matched with the streamlined workflow enabled by TaqMan® MicroRNA Arrays, you can achieve reproducible RT-PCR data that delivers unrivaled sensitivity and specificity across a comprehensive set of miRNA targets derived from the Sanger database.

Also Available in 384-well Arrays

TaqMan® MicroRNA Arrays provide all the advantages of TaqMan® MicroRNA Assays in a convenient, pre-configured 384-well micro fluidic card, reducing experimental variability and the effort required to run 384 assays in parallel.

Assay Workflow

Step 1:
Extract Total RNA		Step 2:
Reverse Transcribe RNA		Step 3:
Set Up Singleplex TaqMan® MicroRNA Assay Reactions
Step 4:
Perform Real-Time PCR Amplification		Step 5:
Analyze Data

  • Applied Biosystems Real-Time PCR System
  • RT-PCR Software
     
30 min65 min25 min90 min 

Array Workflow

Step 1:
Prepare Sample 		Step 2:
Reverse Transcribe		Step 3:
Amplify cDNA (optional)
Step 4:
Dilute cDNA & Load TaqMan® Array		Step 5:
Run TaqMan® Array		Step 6:
Analyze Data


  • Diluted RT product


  • Fast Real-Time PCR System
  • RT-PCR Software
      
30 min 150 min 90 min 10 min 120 min  
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