Robust, Linear miRNA Amplification
Using the NCode™ miRNA Amplification System, you can expect:
- Increased sensitivity with consistent ≥1,000-fold amplification of miRNA
- Amplified product that faithfully represents the initial miRNA sample (Figure 1)
- Reproducibility with precise amplification from reaction to reaction
Figure 1. Sensitive and accurate miRNA microarray profiling from small samples
Total RNA from large-cell carcinomas and adjacent normal tissue was isolated from multiple patients and enriched for miRNA with the PureLink™ miRNA Purification Kit. Enriched miRNA from 300 ng of total RNA was amplified using the NCode™ miRNA Amplification System and labeled for array analysis with the NCode™ miRNA Labeling System. Additionally, the miRNA from 10 µg of total RNA from the same source was labeled with the NCode™ miRNA Labeling System without amplification and hybridized to NCode™ Multi-Species miRNA Microarrays for analysis. The ratios of miRNA expression in tumor samples vs. adjacent normal tissues were calculated for all human miRNA.