Cancer Genotype to Phenotype Research

Navigate the fastest route from cancer genotype to phenotype. Cancer genome projects will soon identify signature sets of oncogenesis-related genes. The next challenge is determining the biological role of each gene and the implication of variation within those genes. Understanding the functional roles of key variants is critical to assess their relevance and to better understand cancer. Life Technologies offers a board portfolio of reagents, instruments, and technologies to help navigate your path from cancer genotype to cancer phenotype.

Gateway® technology is a universal platform that enables you to shuttle your gene of interest (GOI) into as many expression and functional analysis systems as you‘d like. GeneArt® Seamless Cloning and Assembly enables cloning of up to 4 DNA fragments simultaneously into virtually any linearized vector typically in 30 minutes, without extra DNA sequences, restriction endonucleases, or ligation.

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Functionally analyze your cancer genotype of interest through gain- and loss-of-function phenotypes to identify genes and processes regulated by your gene/pathway of interest with our best-in-class transfection and RNAi products. Get superior silencing consistency, potency, and specificity with Silencer® Select siRNAs. Or introduce miRNA precursors or inhibitors directly into cells to up-regulate or down-regulate the activity of specific miRNAs with our soon-to-be-released mirVana™ miRNA mimics and inhibitors.

Find specialized transfection reagents that effectively deliver plasmid DNA, siRNA or duplex RNAi. Perform more transfections with limited or precious cells with Neon® Transfection System—the next-generation electroporation device for highly efficient transfection of primary cells, stem cells, and difficult-to-transfect cells.

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Products   Resources Cancer cell lines transfected with Neon from Protocol Exchange

Choose from a variety of systems designed to expedite your cancer phenotype protein work. Separate proteins easily using Novex® gels. Transfer proteins in just minutes using the iBlot® Dry Blotting System. Quantitate protein activity using Invitrogen™ activity assays with endpoint or real-time analysis.

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Proteins, Expression, Isolation, & Analysis

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Gene regulation in cancer and development is often impacted by epigenetics mechanisms like DNA methylation, chromatin structure, and noncoding RNAs (ncRNAs). These biological processes contribute to a complex regulatory circuit that requires robust tools to study.

  • Detect and quantify DNA methylation efficiently and accurately.
  • Analyze epigenetic modifications and localize proteins to specific regions of genomic DNA.
  • Profile microRNA and ncRNA expression patterns with an optimized platform. Analyze miRNA function with miRNA mimics and inhibitors.

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  • Use the most trusted brand of cell culture media, reagents, cells, and sera:  Gibco® cell culture products—the most widely referenced in scientific literature globally.
  • Detect diverse cancer cell processes in live cells with Molecular Probes® reagents and assays. Many of the assays can be analyzed on a cell-by-cell basis and some are equally suitable for detection with a fluorescence microscope, flow cytometer, or microplate reader.
  • Investigate your cancer phenotype with cancer speciific signaling pathways and signal transduction—everything from high-quality reagents for basic research and assay development to validated biochemical and cell-based assays, and world-class profiling and screening services.

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Genotype to Phenotype Research

 
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