Cancer Gene Expression Analysis

In the study of diseases like cancer, it's not enough to know whether a gene is mutated or normal — it's also important to know whether that gene is expressed and where, when, and what effect that expression has. Whole transcriptome analysis of cancer samples is of growing importance in understanding how altered expression of genetic variants contributes to cancer among other diseases. Analysis of genome-wide differential RNA expression provides researchers with greater insights into biological pathways and molecular mechanisms that regulate cell fate, development, and disease progression.

Methods Used

Next generation sequencing and advances in real-time PCR throughput have revolutionized the way differential expression of genes, including gene alleles and differently spliced transcripts; non-coding RNAs; post-transcriptional mutations or editing; and gene fusions are analyzed.

Currently, the most widely used method to analyze global patterns of gene expression is the DNA microarray. However, because microarrays are hybridization-based, you cannot use them to detect RNA transcripts not included in your array design or from repeated sequences. Also, microarrays offer limited dynamic range to detect subtle changes in expression level of target genes, which is critical in understanding biological response to stimuli or environmental changes.

Discover, profile, & functionally analyze more mRNA and NonCoding RNAs that regulate cancer with sequencing technologies that provide significant advantages over microarray and flexible real-time PCR assay formats for meeting high, medium, and low throughput needs.

Research Need

 

Applications

 

Challenges

  • Identify new RNA cancer biomarkers
  • Profile gene expression levels of known cancer RNA biomarkers in cells or tissues
 
  • Whole transcriptome
  • Gene expression profiling: genome wide or locus-specific on RNA-subpopulations
 
  • Diversity of RNA transcripts
  • RNA instability
  • Ribosomal RNA depletion
 

Life Technologies Solution

Whole Transcriptome

 

Ion RNA-Seq

 

Gene Expression Profiling-RNA Subpopulations

Ion Proton™ Sequencers

 

5500 Series Genetic Analysis Systems

 

See the clear picture. Reveal the diversity and subtleties of the transcriptome with Ion RNA-Seq

 

Ion PGM™ Sequencer

 

QuantStudio™ 12K Flex TaqMan® Assays

Whole Transcriptome Sequencing
Strand specific, hypothesis-free identification and quantification of both known and novel transcripts with a theoretically unlimited dynamic range. Reveal the diversity and subtleties of the transcriptome with Ion RNA-Seq on Ion Proton™ Sequencer or 5500xl SOLiD® Sequencer.

  

Gene Expression Analysis by Real-Time PCR
Determine relative changes in expression across panels of mRNAs and non-coding RNAs without the need to sequence the entire whole transcriptome.
 

miRNA and Small RNA Expression Analysis by Sequencing
Identify and analyze new miRNA and Small RNA expression profiles in cancer samples.

      

All products referenced are for Research Uses Only and are not intended for human or animal therapeutic or diagnostic uses.

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