Stem Cell Reprogramming Tools

Reprogramming somatic cells to induced pluripotent stem cells is a critical and potentially time intensive step in your stem cell research. That’s why Life Technologies offers choices in integration-free reprogramming technologies and services that fit your research and resource needs – you can do it yourself or let us do it for you.

Now available: Essential 6™ Medium for feeder-free reprogramming

Which reprogramming product or service is right for you?

  Episomal iPSC
Reprogramming Vectors*
Epi5™ Episomal iPSC Reprogramming Kit** CytoTune®-iPS Sendai
Reprogramming Kit
CellModel™
Services



Description
Viral-free iPSC generation from normal and diseased cell types
Viral-free iPSC generation from normal and diseased cell types
Efficient generation of integration-free iPSCs from multiple somatic cell types
Reprogrammed cells and world-class support delivered to you
Reprogramming Efficiency 0.002–0.08% 0.04–0.3% 0.01-0.5% 0.01-0.5%
Genes Utilized Thomson/Yamanaka factors Yamanaka factors +Lin28 Yamanaka factors Yamanaka factors
Reprogramming Method & Medium Electroporation using Neon® in N2/B-27 Medium + small molecules Electroporation using Neon® in N2/B-27 Medium Add CytoTune® to cells in fibroblast medium Reprogrammed for you using CytoTune®
iPSC Growth Medium Day 15: Transfer to Essential 8™ Medium Day 15: Transfer to Essential 8™ Medium Day 7: Transfer to Essential 8™ Medium or KnockOut™ Serum Replacement supplemented medium iPSC Medium customizable by project
Genomic
Integration-free
yes yes yes yes
Virus-free
Reprogramming
yes yes
Blood Cell Reprogramming yes yes yes yes
Special
Equipment
Required
Neon® Transfection System or similar device Neon® Transfection System or similar device None None
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*Commercialized in Partnership with Cellular Dynamics International.
**Designed by Dr. Okita in the laboratory of Professor Yamanaka at the Center for iPS Cell Research and Application (CiRA), Kyoto University