StemPro® NSC SFM - Serum-Free Human Neural Stem Cell Culture Medium
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Superior NSC expansion and versatility with serum-free media
- Superior expansion of neural stem cells (NSCs) derived from either embryonic stem cells or from fetal tissue (see Figure 2)
- Versatility to support long-term growth and expansion of both adherent and neurosphere suspension cultures
- Maintain normal NSC multipotency and phenotype/karyotype
- NSCs grown in StemPro® NSC SFM maintain the potential to differentiate to physiologically active neurons and glial cells
- Better batch-to-batch consistency, with each lot produced under cGMP and qualified using an hNSC performance assay
- No or little adaptation required from serum-supplemented medium
hNSCs maintain multipotency and normal phenotype when grown in StemPro® NSC SFM
 Nestin |  Sox2/Oct4 |  Ki67 |
Figure 1. Phenotype marker expression of neural stem cells cultured in StemPro® NSC SFM. Phenotype marker expression of NSC after culture in StemPro® NSC SFM through passage 17. NSCs expressed normal phenotypic markers (Nestin, Sox2) and proliferation marker (Ki67). There was no sign of remnant hESC (Oct4). The inset image in each panel shows the staining pattern given by the nuclear stain DAPI.
StemPro® NSC SFM delivers superior hNSC expansion and versatility for both adherent and neurosphere suspension cultures
StemPro® NSC SFM delivers superior hNSC expansion and versatility for both adherent and neurosphere suspension cultures. As human NSCs comprise a very small fraction of the total CNS cell population, expansion is critical to generate sufficient cells to study differentiation pathways and explore the downstream clinical applications of human NSCs. hNSCs can be passaged only a limited number of times before exhibiting reduced proliferation and differentiation potential. Maximizing the total hNSC yield per passage is therefore essential (Figure 2).
Figure 2. Superior human NSC expansion is achieved using StemPro® NSC SFM compared with competitor serum-free NSC media. StemPro® NSC SFM demonstrates superior cell expansion capacity compared with standard N2-supplemented and competitor neural stem cell media formulations. Proliferation of hNSCs cultured in Invitrogen StemPro® NSC SFM, competitor SCT medium, Sigma medium, and N2 supplemented medium was measured. ESC-derived hNSCs were seeded at 1 x 104 cells per well in Cellstart™ substrate–coated 96-well plates for 3 days in respective media. Indirect cell count was obtained with the CyQUANT® proliferation assay kit (Cat. no. C35006), and data shows mean relative florescence units of stained cells (n=6).
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Figure 2. Superior human NSC expansion is achieved using StemPro® NSC SFM compared with competitor serum-free NSC media. StemPro® NSC SFM demonstrates superior cell expansion capacity compared with standard N2-supplemented and competitor neural stem cell media formulations. Proliferation of hNSCs cultured in Invitrogen StemPro® NSC SFM, competitor SCT medium, Sigma medium, and N2 supplemented medium was measured. ESC-derived hNSCs were seeded at 1 x 104 cells per well in Cellstart™ substrate–coated 96-well plates for 3 days in respective media. Indirect cell count was obtained with the CyQUANT® proliferation assay kit (Cat. no. C35006), and data shows mean relative florescence units of stained cells (n=6).
StemPro® NSC SFM provides the versatility to expand and maintain both adherent and neurosphere suspensions in culture
 (A) Adherent Culture |  (B) Suspension Culture |
Figure 3. Stable proliferation of hNSCs in StemPro® NSC SFM enables culturing of both adherent and suspension culture systems. StemPro® NSC SFM provides the flexibility to culture hNSCs for several passages, maintaining multipotent characteristics as either an (A) adherent or (B) neurosphere culture. hNSCs were derived from hESCs cultured in NSC SFM for 7 passages on CELLstart™ substrate. Tertiary neurospheres were isolated from fetal tissue cultured in NSC SFM.
StemPro® NSC SFM maintains the multipotent differentiation capabilities of hNSCs
hNSCs are defined by the ability to differentiate to three distinct lineages-neurons, oligodendrocytes, and astrocytes. StemPro® NSC SFM delivers a robust, serum-free neural stem cell medium that maintains the multipotent differentiation capabilities of the stem cells and the ability to drive hNSCs down the desired lineage to meet specific experimental requirements (Figure 4).
Figure 4. Differentiation potential of hNSCs cultured in StemPro® NSC SFM. hNSCs were cultured in StemPro® NSC SFM and were differentiated to neurons, and glial cells. Shown above (A) Neurons were labeled with an anti-HuC/D antibody (green) and an anti-Dcx antibody (red). (B) Cells with an oligodendrocyte lineage were labeled with an anti-GalC antibody (red). Cell nuclei were labeled with DAPI (blue) and neurons were labeled with an anti-Dcx antibody (green). (C) Cells with an astrocyte lineage were labeled using an anti-CD44 antibody (green). Cell nuclei were labeled with DAPI (blue) and neurons were labeled with an anti-Dcx antibody (green).
 Panel A |  Panel B |  Panel C |
Figure 4. Differentiation potential of hNSCs cultured in StemPro® NSC SFM. hNSCs were cultured in StemPro® NSC SFM and were differentiated to neurons, and glial cells. Shown above (A) Neurons were labeled with an anti-HuC/D antibody (green) and an anti-Dcx antibody (red). (B) Cells with an oligodendrocyte lineage were labeled with an anti-GalC antibody (red). Cell nuclei were labeled with DAPI (blue) and neurons were labeled with an anti-Dcx antibody (green). (C) Cells with an astrocyte lineage were labeled using an anti-CD44 antibody (green). Cell nuclei were labeled with DAPI (blue) and neurons were labeled with an anti-Dcx antibody (green).
Related products
 | CELLstart™ defined, xeno-free cell culture substrate for stem cells To learn more, visit www.invitrogen.com/stemcell/cellstart |
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