Stem Cell Culture

Since the initial discovery of induced pluripotent stem cells (iPSCs) in 2006, Gibco® media and supplements continue to be widely used for iPSC culture. Knockout™ Serum Replacement is the supplement of choice for iPSC reprogramming, iPSC culture and cryopreservation. For long-term maintenance of pluripotency in feeder-free conditions, choose between StemPro® hESC SFM for standard experiments and KnockOut™ SR XenoFree CTS™ for clinical applications.

Successful human embryonic stem cell (hESC) culture requires standardized and reproducible cell culture conditions for maintenance of hESC pluripotency and long-term cell survival. Whether you require feeder-based, feeder-free, or “cell therapy ready” hESC culture conditions, all Gibco® hESC media are manufactured to cGMP standards, offering the lot-to-to consistency and traceability necessary for reproducible, reliable results.

Life Technologies offers an extensive selection of specialty products optimized for mesenchymal stem cell populations, many of which are free of animal-derived components and backed by our CTS™ brand. These media are designed to minimize adaptation time, maximize cell performance, and help meet regulatory requirements.

Gibco® media and supplements are the most widely cited reagents for use in neural stem cell culture. Choose between the flexibility of our B-27®, N-2, StemPro® Neural supplements and Neurobasal® Medium or ease of use with the StemPro® NSC SFM kit, specifically formulated for serum-free growth and expansion of human neural stem cells.

Hematopoietic stem cells expressing CD34 (CD34+ cells) are defined by their ability to replenish all blood cell types and to self-renew. StemPro®-34 SFM is a serum-free medium specifically formulated to support  the culture of these stem cells, and offers a superior expansion and differentiation of HSCs isolated from bone marrow, peripheral blood, or neonatal cord blood over traditional culture systems.