Sample preparation is a critical step of nearly all genetic studies and stem cells provide an added challenge of limited sample size. With the Ambion® TaqMan® PreAmp Cells-to-CT™, gene expression analysis can be performed directly from limited or small numbers of cultured cells without RNA purification. The protocol includes an intermediate amplification step (pre-amplification) between reverse transcription and real-time PCR in which cDNA is enriched for up to 100 gene targets of the user’s choice without amplification bias.
- Poster: Cardoso, H.A. and Hart, R.P., Establishing standard assays for quality control and identity traceability of human embryonic stem cells (2007)
- White Paper: Eradication of Cross-Contaminated Cell Lines