Protein Expression | Life Technologies

Protein Expression

Protein biomarkers are routinely used to characterize stem cells by cell state, cell type and function. Methods such as immunostaining, Western Blot analysis and FACS have been used to identify, enrich, localize and quantify singular proteins in pluripotent and differentiated cells. However, future needs in stem cell biology require greater throughput and sensitivity to enable global and target-specific analysis. An example of a new target-specific analysis approach is our new TaqMan® Protein Assays.

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Step-by-step Guide to Protein Expression

Identification may be accomplished using the following methods:

Target-Specific Protein Assays, specific markers of pluripotency and differentiation may be monitored
Flow Cytometry, rare populations of differentiated cells among large population of pluripotent cells may be identified

TaqMan® Protein Assays

TaqMan® Protein Expression Assays help quantitate protein expression using gold standard TaqMan® 5' nuclease chemistry with a workflow and sample quantity similar to our all TaqMan® Gene Expression and MicroRNA (miRNA) Assays. As the protein expression results are obtained on the same analytical platform, these revolutionary assays enable direct correlation of mRNA and/or miRNA expression to protein expression. Or, use the TaqMan® Protein Assays Open Kit to make TaqMan® Protein Assay probes using your antibodies.

*Note: Running a complete TaqMan® Protein Assay (pre-designed or made with the Open Kit) requires a Core Reagents Base Kit and Master Mix Kit.

Attune™ Acoustic Focusing Cytometer

The Attune™ Acoustic Focusing Cytometer provides both high sensitivity and high throughput – without sacrificing performance or accuracy. With the Attune™ instrument, you can control your sample concentration, the flow rate, the number of photons you detect, the length of your experiment, the number of samples you run, and more. And you can get the most out of your cells with dependable results. Best of all, our technology makes all of this easy to do.

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Prepare cell lysates for protein assay analysis utilizing the Protein Expression Sample Prep Kit containing the reagents required for the preparation of target and control cell lysates for use with TaqMan® Protein Assay experiments.

Protein Expression Sample Preparation Kit

The Protein Expression Sample Prep Kits enable a gentle, 1-step cell lysis using a buffered non-ionic detergent, with no further sample clean up or purification steps required. The crude lysate samples can be directly mixed with the paired assay probes from any TaqMan® Protein Assay Kit or made using the Open Kit for the target binding step.

*Note: Running a complete TaqMan® Protein Assay (pre-designed or made with the Open Kit) requires a Core Reagents Base Kit and Master Mix Kit.

View ordering information

Characterization may be accomplished using the following methods:

Target-Specific Protein Assays, specific markers of pluripotency and differentiation may be monitored
Flow Cytometry, rare populations of differentiated cells among large population of pluripotent cells may be identified
Western Blot Analysis & Immunostaining, a versatile method to assay for protein markers of pluripotency or differentiation pathways, and could be correlated with gene expression analysis and methylation profiling.