A key area of interest in stem cell biology is understanding the mechanisms that regulate self renewal, differentiation, cell cycle control and other biological processes.
Post-transcriptional regulation by microRNA (miRNA) is one of several mechanisms under investigation. miRNA's are known to regulate the translation and stability of messenger RNAs (mRNA) by cleavage or by blocking the translation of mRNA into protein. Some miRNA's are differentially expressed in stem cells thereby providing unique molecular signatures by biological process, by cell type (embryonic, cancer or tissue-specific cells) and by species.
The Life Technologies miRNA Analysis Workflow is a complete system for miRNA profiling and functional analysis using Real-time PCR. The system offers several benefits over microarray-based solutions:
Experimental Workflow: Find the Products You Need for Every Step
The TaqMan® MicroRNA Cells-to-CT™ Kit is a rapid lysis solution for cultured stem cells (10-1,000,000 cells/sample). It integrates cell lysis, DNA digestion and reverse transcription into a single tube and can be utilized with cells enriched by fluorescence-activated cell sorting (FACS). Alternatively, the mirVana™ miRNA Isolation Kit is designed to isolate total RNA from tissue and primary cells.
Products for Step 1: Isolate miRNA
TaqMan® MicroRNA Cells-to-CT™ Kit
Featuring a unique method for lysing cultured cells while removing genomic DNA and preserving RNA integrity, the TaqMan MicroRNA Cells-to-CT Kit makes it possible to perform microRNA (miRNA) expression profiling directly from cultured cells without RNA purification.
Ambion® mirVana™ miRNA Isolation Kit
Isolate total RNA that contains small RNAs such as miRNA, siRNA, and snRNA. These small RNAs are often lost during traditional RNA isolation methods. In addition, this kit includes reagents and instructions for the isolation of RNA that is enriched for small RNA (<200 bp), enabling more sensitive detection of low-level small RNAs.
- Poster: Cardoso, H.A. and Hart, R.P., Establishing standard assays for quality control and identity traceability of human embryonic stem cells (2007)
- White Paper: Eradication of Cross-Contaminated Cell Lines