Stem Cell Analysis

Sample preparation and purification are critical steps for the success of downstream gene and protein expression studies, and many stem cells provide an added challenge of limited sample size. Choose from a range of scalable and efficient nucleic acid and protein purification technologies and perform gene expression analysis directly from limited or small numbers of stem cells without RNA purification with the TaqMan® PreAmp Cells-to-CT™ Kit.

Life Technologies offers a diverse array of research products for studying stem cell structure, tracing and tracking stem cells, and analyzing proliferation, viability and function. Use Qdot® nanocrystals for live imaging, or choose from the extensive collection of Alexa Fluor® dyes for cell staining. Select from a broad range of fluorescent antibodies for flow cytometry and combine them with the the Attune® Acoustic Focusing Cytometer for true rare event detection in stem cells.

Accurate and sensitive quantification of proteins is key to most stem cell experiments. There are a number of techniques researchers use for quantifying proteins including ELISA, western blot analysis, TaqMan® assays and colorimetric and fluorometric solution assays. Life Technologies offers high-quality, easy-to-use reagents and kits for these methods.

Stem cells are most often identified on the basis of their marker expression. DNA-based methods for the identification of cell lines, assessment of genomic stability, full genome analysis, and detection of adventitious organisms can be applied to stem cells. TaqMan® Arrays can be used to simultaneously analyze the expression levels of 96 genes by quantitative PCR, while the OpenArray® Real-Time PCR Platform can process hundreds to thousands of samples per day.