GIBCO® Rat GPCs

  • Each vial contains 1 x 106 cells isolated from newborn Sprague-Dawley rat cortices
  • Cryopreserved at the end of passage 2 (P2)
  • Minimal variability across lots
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More than 80% of GPCs retain their undifferentiated phenotype

The presence of platelet-derived growth factor AA homodimer (PDGF-AA) and basic fibroblast growth factor (bFGF) in complete GPC growth medium allows rat GPCs to remain undifferentiated.  Figure 1 shows more than 80% of the GPC population retain the undifferentiated phenotypic marker A2B5.


Figure 1.  Rat GPCs stained by indirect immunofluorescence for the cell-surface marker A2B5 (green).  Nuclei were stained with DAPI (blue).  Cells were maintained in the undifferentiated state in GPC recovery media for three days prior to 4% paraformaldehyde fixation and staining.

Components of GPC Recovery Media

 
Product Quantity Catalog Number
StemPro® NSC SFM 1 kit A1050901
GlutaMAX™-1 Supplement 100 mL 35050-061
PDGF-AA Rec Hu 10 µg PHG0035
 

Differentiation of GIBCO® Rat GPCs

Upon expansion, rat GPCs retain their differentiation potential into oligodendrocytes and astrocytes.  Figure 2 shows rat GPCs differentiating into oligodendroctes. More than 30% of rat GPCs were stained positive for GalC (oligodendrocyte specific marker).
 Figure 2.  Rat GPCs differentiating to oligodendrocytes.  The cells were differentiated in growth medium lacking PDGF-AA, EGF, and bFGF, but supplemented with 2% FBS for three days prior to fixation with 4% paraformaldehyde and subsequent staining.  The cell-surface marker GalC was detected by indirect immunofluorescence (green) in ≥30% of the cells in the culture.  Nuclei were stained with DAPI (blue).

Proliferation of GPCs Upon Recovery

GIBCO® rGPCs can be further expanded for at least one passage upon thawing in GPC recovery media (StemPro® NSC SFM, GlutaMAX™-I, and PDGF-AA).  The proliferation abilities afford researchers a more than 2-fold increase in cell numbers while the cells retain their typical morphology (Figure 3).


Figure 3.  Brightfield image of Rat GPCs at passage 3 (P3) that have been cultured in complete StemPro® NSC SFM supplemented with 2 mM GlutaMAX™-I and 10 ng/mL PDGF-AA (i.e., complete GPC growth medium) for three days.  The image was captured using a 10X objective lens.
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