Gibco® Human Neural Stem Cells (H9-Derived)
Ready-to-use human neural stem cells (hNSCs), with superior proliferation and multipotent differentiation potential. Each vial of GIBCO® hNSCs contains >1 × 106 viable cells, derived from NIH-approved H9 (WA09) human embryonic stem cells (hESCs).
Components of the Gibco® Human Neural Stem Cell Kit
- Human neural stem cells (>1 x 106 cells)
- StemPro® NSC SFM Media (KnockOut™ DMEM/F-12, StemPro® NSC SFM supplement, basic FGF recombinant protein, and EGF recombinant protein)
Expansion in adherent cell culture
Figure 1. Phase contrast images (10X) of GIBCO® hNSCs cultured in StemPro® NSC SFM at day 1 (panel A) and at day 3 (panel B) after thawing.
More than 80% of human NSCs retain undifferentiated phenotype
The basic fibroblast growth factor (bFGF) in complete StemPro® NSC SFM allows maintenance of GIBCO® hNSCs in their undifferentiated state. Human NSCs cultured in StemPro® NSC SFM stain positive for the neural stem cell-type specific markers nestin and SOX2, and the proliferation marker Ki67 (>80%) (Figure 2).
|Figure 2. Fluorescence image (20X) of GIBCO® hNSCs at P3 cultured in StemPro® NSC SFM and stained for NSC phenotype markers nestin (green) and proliferation marker Ki67 (red). Cell nuclei were counterstained with DAPI (blue). Approximately 90% of the cells stain positive for the undifferentiated NSC marker nestin and the proliferation marker Ki67. Lack of Oct4 staining indicates no remnant hESCs in the culture.|
Differentiation potential of human NSCs
GIBCO® hNSCs spontaneously differentiate into neurons, oligodendrocytes, or astrocytes, upon withdrawal of bFGF and EGF from culture media. Alternatively, they can be enriched toward a specific lineage upon selection of differentiation medium (Figure 3).
See human NSC spontaneous differentiation protocol
Figure 3. Fluorescence images (20X) of GIBCO® hNSCs cultured in StemPro® NSC SFM for three passages and allowed to differentiate into neurons, oligodendrocytes, or astrocytes. Upon directed differentiation, cells start to lose the undifferentiated NSC marker nestin, but stain positive for differentiated cell type markers Dcx, GalC, and GFAP. Cells were stained for the undifferentiated NSC markers nestin (red) and SOX2 (green) prior to directed differentiation (panel A). Cells were then differentiated into neurons and glial cells, and respectively stained for neuronal marker Dcx (green) (panel B), oligodendrocyte marker GalC (red) (panel C), or for astrocyte marker GFAP (green) (panel D). Nuclei were counterstained with DAPI (blue) in panels B–D.