Pluripotent Stem Cell (PSC) Culture Media
4 Media Options, 20+ Protocols, and 50 Years of InnovationPluripotent stem cell (PSC) research requires careful attention to culture conditions. To find the right solution for the right environment, tap into peer-reviewed publications, protocols, instructional videos and stem cell experts. From KnockOut™ Serum Replacement to Essential 8™ Medium and cell therapy research media, Gibco® products deliver culture with confidence. |
Which Pluripotent stem cell culture medium is right for you?
| The standard for serum-free, feeder-based culture of ESCs and iPSCs from multiple species | Feeder-free culture with flexibility to optimize bFGF concentration and maintain differentiation capacity of PSCs | Optimal feeder-free culture of all human ESC and iPSC lines with minimal lot-to-lot variability | Fully documented xeno-free PSC culture reducing the burden in qualifying reagents, facilitating transition, from the bench to the clinic | ||||
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Product name |
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| Recommended applications | Feeder culture; reprogramming on feeders; differentiation of PSCs | Feeder-free culture | Feeder-free, xeno-free culture; feeder-free reprogramming | Feeder-based and feeder-free xeno-free culture for cell therapy research | |||
| Recommended PSC species | Multiple species | Human | Human | Human | |||
| Lot-to-lot variability |
Medium | Medium | Low | Medium | |||
| Cell morphology | Compact colonies with smooth edges. High nuclear to cytoplasmic ratio | Compact colonies with smooth edges. Some colonies will have undefined borders with spiked edges | Compact colonies with smooth edges. High nuclear to cytoplasmic ratio | Compact colonies with smooth edges. Some colonies will have undefined borders with spiked edges | |||
| Components/unit |
1 x 100mL or 500mL |
1 x 10ml StemPro® Supplement
1 x 500ml DMEM/F12 with GlutaMax™ 1 x 40ml 25% BSA |
1 x 10ml Essential 8™ Supplement
1 x 500ml DMEM/F12 with GlutaMax™ |
1 x 100ml KnockOut™ SR XenoFree CTS™
1 x 500ml KnockOut™ DMEM CTS™ 1 x 5ml KnockOut™ GF Cocktail CTS™ |
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| Additional components required for complete medium |
D-MEM/F-12 (1X) with GlutaMAX™-I , NEAA, bFGF, 2-mercaptoethanol. For cost-effective option, choose the KnockOut™ ESC/iPSC Media |
bFGF and 2-mercaptoethanol | No additional components required | bFGF CTS™ and 2-mercaptoethanol | |||
| Price/500 ml of complete medium |
$$ (individually)
$ (with kit) |
$$ | $ | $$$$ | |||
| Stability of complete medium | Stable at 4°C for 2 weeks | Stable at 4°C for 2 weeks | Stable at 4°C for 2 weeks | Stable at 4°C for 2 weeks | |||
| Specifically designed for cell therapy research |
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| Available Resources |
Protocols for culturing PSCs, publications, hands-on training, product finder |
Protocols for culturing PSCs |
Protocols for culturing PSCs, FAQs, hands-on training |
Protocols for culturing PSCs, FAQs, product documentation |
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| Price/unit | $60.00-$283.00 | $257.00 | $195.98 | $391.00-$2,045.00 | |||
| Order Now | Order Now | Order Now | Order Now |
