Induced Pluripotent Stem Cell Media & Reagents

GIBCO® media and reagents have been at the forefront of pluripotent stem cell research for years.  Knockout™ DMEM supplemented with Knockout™ Serum Replacement is the media of choice for embryonic stem cell growth and now iPS cell culture.(3-9)  In addition, StemPro® hESC SFM can be used for feeder-free iPSC culture and the new Knockout™ SR XenoFree for feeder-free, xeno-free culture of iPSCs.
Figure 1 - Knockout™ DMEM supplemented with Knockout™ Serum Replacement is the media of choice for embryonic stem cell and now iPS cell culture.

Together, our substrates, freezing media, and growth factors offer a complete portfolio of cell culture tools for iPS cell selection and expansion.

New Publication on Ideal xeno-free culture conditions for iPSC generation

Rodríguez-Pizà I, et. al "Reprogramming of Human Fibroblasts To Induced Pluripotent Stem Cells Under XenoFree Conditions". Stem Cells, 2009 Nov 3. [Epub ahead of print]

Important advances in this paper:
  • Derivation of iPSCs and clonal expansion of colonies under xeno-free conditions
  • iPSCs derived in xeno-free conditions differentiated to beating cardiomyocytes
  • iPSC lines tested to be karyotypically normal (46XY) at Passage 10

Learn more about GIBCO® substrates for iPS cells

Learn more about GIBCO® media for iPS cells