Lentiviral iPSC Reprogramming Particles

  • Lentiviral reprogramming particles for creating iPSCs
  • Lentiviral constructs include hOct4, hSox2, hKlf4, hc-Myc, hLin28, hNanog, hOct4-GFP reporter, and CMV-GFP reporter
  • Ready-to-use viral particles help save time
  • Lentiviral technology provides efficient gene delivery

Produce Pluripotent Stem Cells

Invitrogen™ lentiviral reprogramming particles can be used to convert somatic cells into induced pluripotent stem cells (iPSCs) as described in the literature [1–3].

Selection Guide

With six induction genes and two reporter genes to choose from, you have options for following various published protocols.
Takahashi K et al. [1] Oct4, Sox2, Klf4, c-Myc
Yu J et al. [2] Oct4, Sox2, Nanog, Lin28
Transduction Reporter CMV-GFP Reporter
Pluripotency Reporter Oct4-GFP Reporter

Figure 1: Precise Control of Expression Delivers Flexibility

In the presence of Tetrepressor, expression of the gene of interest—in this case, Oct4—is effectively turned off. Adding tetracycline reverses this repression, allowing precise control of the expression level (Figure 2). HT1080 cells were transduced with or without Lenti6.3-TetR virus particles (available through Invitrogen Custom Services) at various MOIs (1, 5, 10, and 30), followed by transduction with or without Lenti6.3-OCT4 virus at a multiplicity of infection (MOI) of 5 as indicated. UntransducedHT1080 cells were used as a negative control. Panel A shows western blot analysis of Oct4 protein expression (top) and TetR protein (bottom). Lane 1: Positive controls, cells transfected with pLenti6.3 Oct4 (top) and pLenti6.3 tetR(bottom) plasmids; Lane 2: Mock-transduced cells; Lane 3: Cells transduced with Lenti6.3-OCT4 virus as a control for Oct4; Lane 8: Cells transduced with Lenti6.3-TetR virus as a control for tetR. Panel B shows relative expression levels of each protein, based on band intensities shown in Panel A, compared to that of Oct4 control (Lane 3) and tetR control (Lane 8) as 100%.

References

  1. Takahashi K et al. Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors. (2007) Cell 131:861–872.
  2. Yu J et al. Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. (2007) Science 318:1917–1920.
  3. Park I-H. Generation of human-induced pluripotent stem cells. (2008) Nature Protocols 3:1180–118
For Research Use Only.  Not intended for animal or human therapeutic or diagnostic use.