Reduce Hands-on Time with the CytoTune®-iPS Sendai Reprogramming Kit
Click on the tabs below to find out how you can experience successful reprogramming in one simple transduction.
Comparison of reprogramming workflows using the CytoTune®-iPS Sendai Kit, mRNA, and lentivirus
Protocol timeline for iPSC generation from human primary fibroblasts with the CytoTune®-iPS Sendai Reprogramming Kit
A single transduction with the CytoTune® kit generates iPSC colonies typically 3-4 weeks post-transduction. For a detailed protocol, please consult the product handbook.
|Day -2||Plate fibroblasts in fibroblast culture medium (DMEM + 10% FBS) into two wells of a 6-well plate to achieve 5 x 105 cells/well on day 3.|
|Day 0||Add CytoTune®-iPS Reprogramming Kit contents to your cells.|
|Day 1-7||Replace the fibroblast culture medium (DMEM + 10% FBS) with fresh fibroblast culture medium on day 4, and then every other day thereafter.|
|Day 7||Harvest cells with trypsin, then plate the cells onto MEF feeder-coated plates in fibroblast culture medium.|
|Day 8||Replace the fibroblast medium with complete KnockOut™ Serum Replacement medium.|
|Day 9-25||Observe the cells and replace the spent medium daily. Look for the emergence of cell clumps indicative of iPSCs.|
|3-4 weeks post-transduction||Perform live staining with Tra1-60 or Tra1-81 to select reprogrammed colonies. Manually pick colonies and transfer them to fresh MEF plates.|
Simple generation of iPSC colonies from human neonatal foreskin fibroblast cells (strain BJ) with the CytoTune®-iPS Sendai Reprogramming Kit
Learn more about the CytoTune® kit
For research use only. Not for human or animal therapeutic or diagnostic use.