Passaging Pluripotent Stem Cells
Pluripotent Stem Cell passaging or dissociating can be accomplished by a number of different methods depending on your culture medium and application.
Whether you prefer to passage your cells enzymatically or manually, Gibco® media provides you with choices for PSC subculture.
Which passaging method is right for you?
|Recommended for clump passaging in feeder and feeder-free cultures||Standard reagent for clump passaging in feeder and feeder-free cultures||Quick and gentle dissociation, recommended for single cell passaging||Recommended for clump passaging with feeder-free Essential 8™ Medium||Optimal for clinical research applications that require human origin materials only||Quick and easy manual passaging tool for feeder and feeder-free cultures|
|Recommended applications||Passaging in clumps||Passaging in clumps||Dissociation to single cells||Passaging in clumps||Passaging in clumps or single cell passaging||Mechanical passaging in clumps|
|Source origin||Bacillus polymyxa||Clostridium histolyticum||Marine||Chemical animal-origin free||Recombinant enzyme animal-origin free||Not applicable|
|Form||Lyophilized powder||Lyophilized powder||Liquid, ready to use||Liquid, dilute 1,000x||Liquid, ready to use||Individually wrapped|
|Storage Temperature||2-8°C||2-8°C||2-8°C||Room temperature||Room temperature||Room temperature|
|Shelf life of liquid format||2 weeks||2 weeks||2 years||2 years||2 years||Not applicable|
|Inactivation method||Inhibition by dilution (no inhibitors needed)||Inhibition by dilution (no inhibitors needed)||Inhibition by dilution (no inhibitors needed)||Inhibition by dilution (no inhibitors needed)||Inhibition by dilution (no inhibitors needed)||Not applicable|
|Unit size||5 g||1 g||100 mL||4 x 100 mL||100 mL||Box of 10 units|
|Optimized concentration for use||2mg/mL||
1mg/mL for feeder-dependent,
10mg/mL for feeder-free
|1X Solution||0.5 mM||1X Solution||Not applicable|
|Number of plates passaged/unit||1,250 x 60mm plates||
500 x 60mm plates at 1mg/mL,
50 x 60mm plates at 10mg/mL
|50 x 60mm plates||50,000 x 60mm plates||50 x 60mm plates||10 plates|