Passaging Pluripotent Stem Cells
Pluripotent Stem Cell passaging or dissociating can be accomplished by a number of different methods depending on your culture medium and application. Whether you prefer to passage your cells enzymatically or manually, Gibco® media provides you with choices for PSC subculture. |
Which passaging method is right for you?
| Recommended for clump passaging in feeder and feeder-free cultures | Standard reagent for clump passaging in feeder and feeder-free cultures | Quick and gentle dissociation, recommended for single cell passaging | Recommended for clump passaging with feeder-free Essential 8™ Medium | Optimal for clinical research applications that require human origin materials only | Quick and easy manual passaging tool for feeder and feeder-free cultures | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
Product name |
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| Recommended applications | Passaging in clumps | Passaging in clumps | Dissociation to single cells | Passaging in clumps | Passaging in clumps or single cell passaging | Mechanical passaging in clumps | |||||
| Source origin | Bacillus polymyxa | Clostridium histolyticum | Marine | Chemical animal-origin free | Recombinant enzyme animal-origin free | Not applicable | |||||
| Form | Lyophilized powder | Lyophilized powder | Liquid, ready to use | Liquid, dilute 1,000x | Liquid, ready to use | Individually wrapped | |||||
| Storage Temperature | 2-8°C | 2-8°C | 2-8°C | Room temperature | Room temperature | Room temperature | |||||
| Shelf life of liquid format | 2 weeks | 2 weeks | 2 years | 2 years | 2 years | Not applicable | |||||
| Inactivation method | Inhibition by dilution (no inhibitors needed) | Inhibition by dilution (no inhibitors needed) | Inhibition by dilution (no inhibitors needed) | Inhibition by dilution (no inhibitors needed) | Inhibition by dilution (no inhibitors needed) | Not applicable | |||||
| Unit size | 5 g | 1 g | 100 mL | 4 x 100 mL | 100 mL | Box of 10 units | |||||
| Optimized concentration for use | 2mg/mL |
1mg/mL for feeder-dependent, 10mg/mL for feeder-free |
1X Solution | 0.5 mM | 1X Solution | Not applicable | |||||
| Number of plates passaged/unit | 1,250 x 60mm plates |
500 x 60mm plates at 1mg/mL, 50 x 60mm plates at 10mg/mL |
50 x 60mm plates | 50,000 x 60mm plates | 50 x 60mm plates | 10 plates | |||||
| Unit price | $526.00 | $212.00 | $36.94 | $62.00 | $35.87 | $193.00 | |||||
| Order | Order | Order | Order | Order | Order |
