Extracellular Matrices for PSC Culture

We provide extracellular matrices (ECMs) that are designed to minimize adaptation time, maximize cell performance, and help meet regulatory requirements.

Whether you are culturing your cells on feeder layers or require feeder-free xeno-free conditions for more clinical applications, Gibco® products provide you with all the choices to culture your pluripotent stem cells (PSC) in the right environment.

Which extracellular matrix is right for you?

  Most commonly used basement membrane matrix for ESC and iPSC cultures Optimized for use with Essential 8™ Medium Optimal for clinical research applications that require human origin materials only

Product name
Recommended applications Feeder and feeder-free culture Feeder-free and xeno-free culture Feeder-free and xeno-free culture
Recommended medium pairing StemPro® hESC SFM Essential 8™ Medium KnockOut™ SR XenoFree CTS™-supplemented medium
Source origin Cultured mouse EHS tumor cells;
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Human (recombinant);
defined
Blend of human native proteins;
defined
Storage temperature Frozen (-20 to -80° C) Frozen (-20 to -80° C) Refrigerate (2-8°C)
Plate preparation time 1 hr incubation at 37°C 1 hr incubation at room temperature 1-2 hrs incubation at 37°C
Shelf life of prepared plate 2 weeks at 2–8°C 1 week at 2-8°C 1-7 days at 2-8°C (tightly sealed)
Unit Size 1 mL or 5 mL 1 mL 2 mL
Number of plates coated/unit 16 x 6-well plates
from 1 mL
vial diluted 1:100
16 x 6-well plates
from 1 mL
vial diluted 1:100
22 x 6-well plates
when diluted 1:50
Price per unit $44.29-$201.00 $49.25 $236.00
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Attachment Factor (0.1% Gelatin, Cat. No. S006100) may be used for feeder-layer attachment.