Essential 6™ Medium and Essential 8™ Medium

When used together, Essential 6™ Medium and Essential 8™ Medium streamline the generation, expansion, and differentiation of pluripotent stem cells (PSCs) by offering a feeder-free, xeno-free system for all three steps.

Originally developed and published by Guokai Chen et al.1 in the laboratory of James Thomson, Essential 8™ Medium contains only the essential components needed for stem cell culture*. Essential 6™ Medium uses the same base formulation as Essential 8™ Medium, but without TGFβ or bFGF to allow for reprogramming and differentiation.

  • Want to learn more about adopting Essential 6 and Essential 8 media into your stem cell culture system? Send us an inquiry.
Expansion Differentiation Reprogramming
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Want to learn more about using Essential 6™ Medium and Essential 8™ Medium in your stem cell research, send us an inquiry.

Essential 8™ Medium and Essential 6™ Medium are commercialized in partnership with Cellular Dynamics International.

 

Citation:

1Chen G, Gulbranson DR, Hou Z, et al. (2011) Chemically defined conditions for human iPSC derivation and culture. Nat Methods Apr 10.

* Supports growth for >50 passages without any signs of karyotypic abnormalities

For research use only. Not for use in diagnostic procedures.

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