Custom Panels

Ion AmpliSeq™ Custom DNA Panels

Minutes to design Human
or Mouse DNA panels

Simplicity	Scalability	Speed
Customize with your genes	12 to 6,144 amplicons per pool	Just minutes to submit design
10 ng of input DNA	One to hundreds of genes	Automated primer design in 2 hours
Simple PCR-based target selection	96 barcodes for multiplexing	Targeted library constructed in 3.5 hours

If the Ion AmpliSeq™ Ready-to-use Panels don’t match your gene targets then simply select a subset of the targets or simply enter your specific genomic content online and we will design and create customized Ion AmpliSeq™ Panels just for you.

Creating and ordering custom panels is easy with the Ion AmpliSeq™ Designer—a free, online assay design tool that connects you directly to our assay design pipeline. It's developed, optimized, and proven for human gene sequencing. All you need is a list of HUGO gene symbols or gene regions based on hg19 coordinates. Register at the Ion Community to use this solution.

Custom panels are delivered in two formats: tubes of pre-pooled multiplexed primers in ready-to-use concentration and 384-well plates in which each well contains individual primer pairs in concentrated format. For custom panels with ≤96 primer pairs, you will receive 2,250 total reactions. For custom panels with >96 primer pairs, you will receive 9,000 total reactions.

Ion AmpliSeq™ Designer Performance Specifications

Specifications*	Current value
Input DNA amount per primer pool	10 ng
Primer Pairs per pool	12 to 6,144
Target size	1 kb to 5 Mb
Target design rate	>90%
Coverage uniformity	>85%

*Performance values will vary with each custom design
Target design rate—bases covered by the insert region of amplicons out total target bases submitted
Typical coverage uniformity—bases covered at > 20% of the mean coverage