Ion AmpliSeq™ Custom DNA Panels
|Customize with your genes||12 to 6,144 amplicons per pool||Just minutes to submit design|
|10 ng of input DNA||One to hundreds of genes||Automated primer design in 2 hours|
|Simple PCR-based target selection||96 barcodes for multiplexing||Targeted library constructed in 3.5 hours|
If the Ion AmpliSeq™ Ready-to-use Panels don’t match your gene targets then simply select a subset of the targets or simply enter your specific genomic content online and we will design and create customized Ion AmpliSeq™ Panels just for you.
Creating and ordering custom panels is easy with the Ion AmpliSeq™ Designer—a free, online assay design tool that connects you directly to our assay design pipeline. It's developed, optimized, and proven for human gene sequencing. All you need is a list of HUGO gene symbols or gene regions based on hg19 coordinates. Register at the Ion Community to use this solution.
Custom panels are delivered in two formats: tubes of pre-pooled multiplexed primers in ready-to-use concentration and 384-well plates in which each well contains individual primer pairs in concentrated format. For custom panels with ≤96 primer pairs, you will receive 2,250 total reactions. For custom panels with >96 primer pairs, you will receive 9,000 total reactions.
Ion AmpliSeq™ Designer Performance Specifications
|Input DNA amount per primer pool||10 ng|
|Primer Pairs per pool||12 to 6,144|
|Target size||1 kb to 5 Mb|
|Target design rate||>90%|
*Performance values will vary with each custom design
Target design rate—bases covered by the insert region of amplicons out total target bases submitted
Typical coverage uniformity—bases covered at > 20% of the mean coverage
For research use only. Not intended for any animal or human therapeutic or diagnostic use.