Ion AmpliSeq™ RNA Panels
gene expression analysis
Ion AmpliSeq™ technology delivers simple and fast library construction, for affordable targeted sequencing of specific human genes. Ion AmpliSeq™ RNA Panels enable scientists to choose from over 20,000 targeted genes to survey gene expression fold changes in samples requiring as little as 500 pg of unfixed RNA or 5 ng of FFPE RNA. The Ion PGM™ System, coupled with Ion AmpliSeq™ RNA Panels, Torrent Suite™ Software and Partek Flow® Software, enable a unique and complete workflow solution.
Confirm expression using TaqMan® AssaysUniversal Human Reference (UHR) RNA and Human Brain Reference (HBR) RNA control samples were used to compare fold-change for each of the 50 genes included in the Ion AmpliSeq™ RNA Cancer Panel against the fold change calculations of the same genes with TaqMan® Gene Expression Assays, resulting in a correlation of 0.989, as shown in Figure 1. These results show the accuracy of the gene expression data obtained with the Ion AmpliSeq™ RNA workflow. Find TaqMan® Assays corresponding to the same gene regions interrogated by Ion AmpliSeq™ RNA amplicons in the Ion Community.
The 1.5 day workflow from RNA to annotated genes produces targeted libraries in 5.5 hours, after which template is prepared using the Ion One Touch™ 2 System and then sequenced on the Ion PGM™ Sequencer. Automated analysis is then performed with Torrent Suite™ Software, while additional analysis can be performed using Partek Flow® software.
Figure 2. Ion AmpliSeq™ RNA Cancer Panel workflow using an Ion 318™ Chip and 4.5 hour, 1 x 200 base sequencing run.
Ion AmpliSeq™ RNA Cancer Panel
The Ion AmpliSeq™ RNA Cancer Panel was developed as a RNA complement to the Ion AmpliSeq™ Cancer Hotspot Panel v2. The panel is a single pool of primers targeting 50 oncogenes and tumor suppressor genes including KRAS, BRAF, and EGFR genes.
Ion AmpliSeq™ RNA Apoptosis Panel
The Ion AmpliSeq™ Apoptosis Panel is a screening tool designed against 267 genes involved in the cellular apoptosis pathway. Included are genes associated with death receptor– mediated apoptosis, c-myc, and p53-mediated apoptosis.
Custom AmpliSeq™ RNA Panels
Ion AmpliSeq™ Designer is a free online tool that allows researchers to create and order Ion AmpliSeq™ RNA Custom Panels comprising just their genes of interest. Leveraging more than a decade of expertise powering the TaqMan® Custom Assay design pipeline, Ion AmpliSeq™ Designer produces optimized primer designs in just hours. You now have full flexibility to analyze hundreds of genes of your choice, such as those implicated in a particular disease state or representing specific biochemical pathways.Design and order your panel
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. The content provided herein may relate to products that have not been officially released and is subject to change without notice.