Ion AmpliSeq™ Target Selection Technology
Transformative Target Selection Technology
Simplicity
• Convenient ready-to-use or custom panels• Just 10 ng of input DNA—enables routine sequencing of FFPE samples
• Simple PCR-based target selection—no special equipment needed
Scalability
• Up to 1,536 custom or 3,000 ready-to-use amplicons per tube• Up to 96 barcodes for sample multiplexing
• Three Ion Chips producing from 10 Mb and up to ≤1 Gb of total sequence
Speed
• Automated primer design for custom panels in as little as 2.5 hours with minutes of hands-on time• Targeted library constructed in 3.5 hours
• Go from DNA to variants in a single day with a 2-hour sequencing run
Get started with convenient, Ion AmpliSeq™ Ready-to-use Panels or allow us to create custom panels for you, using the Ion AmpliSeq™ Designer online tool.
Ion AmpliSeq™ technology delivers simple and fast library construction for affordable targeted sequencing of specific human genes or genomic regions. Based on ultrahigh-multiplex PCR, Ion AmpliSeq™ technology requires as little as 10 ng of input DNA to target sets of genes, making sequencing of FFPE samples routine on Ion PGM™ Systems.
Alternative target selection methods are lengthy and complex and require large amounts of DNA, making them impractical for sequencing DNA from the formalin-fixed, paraffin-embedded (FFPE) tissues that are commonly used in translational and clinical research. Using just 10 ng of FFPE DNA and one pool of 1,536 amplicons, variants can be produced in a single day using the Ion AmpliSeq™ Custom workflow. By contrast, the same project using alternative workflows requires 100 times as much DNA, 4 times the pools, and twice the time.
Ion PGM™ Sequencer Workflow
Ion Ampliseq™ Primer Pool and 10 ng DNA
The Ion OneTouch™ System for template preparation is fully automated and requires just minutes of hands-on time
The Ion PGM™ Sequencer is ideal for sequencing small genomes, sets of genes, or performing gene expression profiling or ChIP-Seq. Using Ion AmpliSeq™ Custom Solutions, researchers can interrogate targeted genomic regions using up to 1,536 amplicons in a single tube, in a single day
Torrent Suite analysis software delivers significant improvement in base-call accuracy while cutting analysis time to a minimum
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