Small RNA & miRNA Sequencing on the 5500 Genetic Analyzer

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Overview of SOLiD® Total RNA-Seq Kit Workflow

The SOLiD^® Small RNA Solution is a robust method for hypothesis-neutral, whole genome analysis of expression patterns that enables you to discover novel RNA without the probe bias of microarrays. Using the SOLiD^® Total RNA-Seq Kit, you can convert total RNA into a library in a single day. This simplified protocol reduces experimental variability and improves the detection of biologically relevant changes.

The SOLiD^® Total RNA-Seq kit is a single kit for the construction of libraries either for small RNA or whole transcriptome analysis.

• Conserve strand specificity of cDNA—allowing you to know exactly which strand a particular small RNA originated from
• Offer better sensitivity—high number of mapped reads coupled with the accuracy of exact call chemistry (ECC) give confidence in detecting rare and low-abundance small RNAs
• Offer versatile inputs—analyze small RNAs from a variety of sample types, including quality compromised samples, such as RNA from FFPE tissue, starting with as little as 5 ng   Learn More
• Detect novel RNAs—facilitate detection of unmapped small RNAs and isoforms
• Allow multiplexing—sequence multiple small RNA libraries simultaneously in a single lane, helping to reduce the cost of analysis per sample