RNA Sequencing

A detailed understanding of the RNA content (coding and non-coding) in a given cell or cell sample is invaluable for the understanding of differential expression in normal and disease processes. Microarrays have historically been used for RNA profiling; however, this technique suffers from significant shortcomings, such as low specificity, sensitivity, and dynamic range, which reduce accuracy. Perhaps more importantly, microarrays restrict expression profiling results to specific annotations and content.

RNA Sequencing approaches avoid these weaknesses to give a more quantitative view of the RNA population within a cell by providing higher sensitivity and a broader dynamic range—and a hypothesis-neutral approach. Interrogate a cell’s whole transcriptome, small RNA content, or gene expression profile, all on the sequencing platform of your choice.