Targeted Resequencing Sample Prep

Resequencing experiments are fundamental in basic and clinical research as scientists look for causative mutations within populations. Targeted resequencing involves the comparative analysis of candidate genes or regions and requires a high level of accuracy to identify low frequency SNPs and structural variants. A large number of genes must be analyzed to elucidate such variations, making next generation sequencing an attractive solution.

Step-by-Step Guide to Targeted Resequencing Sample Prep Products

All Next Generation Sequencing Platforms

PureLink™ Genomic DNA Mini Kit
Recommended for next generation sequencing applications requiring extraction and purification of genomic DNA samples. Yields high-quality DNA in a single column.

PureLink™ Genomic DNA Mini Kit

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All Next Generation Sequencing Platforms

SequalPrep™ System
Robust target enrichment by long PCR (5-20kb) for enhanced sequencing of targeted regions, followed by one-step amplicon purification and input equalization in a convenient plate for accurate target representation.

Cot-1DNA® Blocking Reagent
Increase hybridization sensitivity on array-based exon capture platforms by blocking repetitive sequences.

SequalPrep™ & Cot-1 DNA Blocking Reagent

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SOLiD® Applications

SOLiD® Fragment Library Construction
Fully optimized complete set of reagents, columns, and size selection gels to generate fragment libraries for use on SOLiD® System.

SOLiD® Mate-Paired Library Construction
Fully optimized, validated, complete set of reagents to construct mate-paired libraries for use on SOLiD® System.

All Next Generation Sequencing Platforms

E-Gel™ SizeSelect™ Gels
Rapid size separation and recovery system for library construction.

SOLiD® Applications & All Next-Gen Sequencing Platforms

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SOLiD® Applications

SOLiD® Library TaqMan® Quantitiation Kit
Enables measurement of amplifiable template in a SOLiD® library for optimal input in emulsion PCR (ePCR).

SOLiD® Library TaqMan® Quantitation Kit

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SOLiD® Applications

SOLiD® Fragment Library Barcoding Kits
Unique barcode sequences for SOLiD® fragment libraries to sequence multiple samples on one slide. Increase throughput and efficiency while cutting cost.