Learn Wildfire technology and the promise it holds for simplifying the workflow and improving the throughput of the 5500 Series Genetic Analyzers.

Three sessions focused on LifeScope™ Software. Topics include:

• NGS-227-1 XSQ File Format and XSQ Tools (25 minutes)
• NGS-227-2 Intro to LifeScope™ Software, Optimal Analysis for 5500 Series SOLiD™ Data
• NGS-227-3 BioScope™ to LifeScope™ Software Transition Training

NGS-226 -1 XSQ File Format and XSQ Tools (25 minutes)

The advent of ultrahigh-throughput sequencing has introduced new and boosted the capabilities of older methodologies. This training will demonstrate how the SOLiD™ System has been applied to cancer research by focusing on published cancer research using on the SOLiD™ System. The session will cover resequencing, targeted and whole genome sequencing transcriptome and miRNA analysis.

Two sessions focused on de novo assembly of small genomes using the SOLiD™ System. Topics include:

• NGS-223-1 Assembly of small genomes featuring SOLiD™ De Novo accessory tools (40 minutes)
• NGS-223-2 SOLiD™ De Novo Assembly of Small Genomes: Case Studies of Listeria and Salmonella (35 minutes)

Five sessions focused on SOLiD™ epigenomics. Topics include:

• NGS-222-1 SOLiD™ ChIP-Seq Application: the Safest Way to Find Out What’s Touching Your DNA (24 minutes)
• NGS-222-2 DNA Methylation Analysis on the SOLiD™ System: Read the Code Behind the Sequence (38 minutes)
• NGS-222-3 SOLiD™ Epigenomics Data Analysis Featuring CLC bio (20 minutes)
• NGS-222-4 SOLiD™ Epigenomics Data Analysis Featuring Partek (20 minutes)
• NGS-222-5 DNA Methylation Data Analysis on the SOLiD™ System (41 minutes)

Four sessions focused on the Targeted ReSeq application, covering the end-to-end workflow, from how to design a Targeted ReSeq experiment to performing data analysis. Topics include:

• NGS-221-1 TargetSeq™ on the 5500 Series Genetic Analyzer and SOLiD™ 4 (42 minutes)
• NGS-221-2 SOLiD™ Targeted ReSeq Data Analysis Featuring CLC bio (20 minutes)
• NGS-221-3 SOLiD™ Targeted ReSeq Data Analysis Featuring SoftGenetics (20 minutes)
• NGS-221-4 SOLiD™ Targeted ReSeq Data Analysis Featuring Geospiza (20 minutes)

"Nine sessions focused on the RNA-seq application, covering the end-to-end workflow from how to design an RNA-seq experiment up to how to perform data analysis. You will learn: what are the important questions you need to ask before defining which type of study to perform; tips & tricks to be successful during library prep; the power of barcoding; and how to perform data analysis using Life Technologies and third Party tools. Topics include:

• NGS-220-1 So You Want to Do RNA Sequencing Using the SOLiD™ System; Now What? (29 minutes)
• NGS-220-2 Step-by-Step Ooverview of the STaR Kit Protocol (39 minutes)
• NGS-220-3 Unlocking the Potential of RNA-Seq With External RNA Controls (46 minutes)
• NGS-220-4 SOLiD™ RNA-Seq Data Analysis Featuring BioScope™ Software (30 minutes)
• NGS-220-5 SOLiD™ RNA-Seq Data Analysis Featuring CLC bio (20 minutes)
• NGS-220-6 Data analysis featuring Partek (20 minutes)
• NGS-220-7 SOLiD™ RNA-Seq Data Analysis Featuring Geospiza (20 minutes)
• NGS-220-8 SOLiD™ RNA-Seq Data Analysis Featuring SoftGenetics
• NGS-220-9 SOLiD™ RNA-Seq Data Analysis Featuring TopHat and Cufflinks (20 minutes)
• NGS-220-10 Barcoding SOLiD RNA Libraries 101 (44 minutes)

Dr Rebecca J Leary from the Ludwig Center for Cancer Genetics and Therapeutics at Johns Hopkins Kimmel Cancer Center

Dr Stanley F Nelson from the Department of Human Genetics at David Geffen School of Medicine at UCLA

Dr John Carpten from the Division of Integrated Cancer Genomics at TGen

Dr Alex Hoischen from Nijmegen

Dr Kai Q Lao, Principal Scientist at Life Technologies