Next-Generation Sequencing Systems, Accessories & Reagents

The 5500 W Series Genetic Analysis Systems - scalable and accurate next generation sequencing.

The 5500 W Series Genetic Analysis Systems support a wide range of applications on a per-lane basis with independently addressable and configurable lanes on either one or two FlowChips. The Exact Call Chemistry (ECC) module boosts the already industry leading accuracy of this ligation-based sequencing.

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5500 W Series Genetic Analyzer V1.0 Specifications

System and Features	5500 W System (1 FlowChip)	5500xl W System (2 FlowChips)
Pay-Per-Lane Sequencing	Reagent consumption engineered independently for each lane; users pay only for the consumables in the active lanes when performing a partial run.
Application-Per-Lane Sequencing	Independent FlowChip lanes allow users to configure read length of chemistry for each lane enabling multiple applications in a single run.
Multiplexing	96 barcodes for both RNA and DNA applications.
Instrument Throughput¹ 1 x 50 1 x 75 2 x 50 MP	~ 80 Gb ~ 120 Gb ~ 160 Gb	~ 160 Gb ~ 240 Gb ~ 320 Gb
Exomes/run²	Up to 18	Up to 36
Transcriptomes/run³	Up to 12	Up to 24
Small RNA/run⁴	Up to 144	Up to 288
Maximum Read Lengths⁵	1 x 75 Frag, 2 x 50 MP

Instrument throughput is estimated using typical runs at a density of 700,000/panel, 65%-70% passing filter. Actual throughput will depend on the samples being run. Throughput uses the quality reads from the instrument that pass the primary analysis filter.
~50 Mb exome, >100x average coverage using fragment chemistry, 1 x 75 bp run.
Transcriptome assumes > 100 million reads/sample using fragment chemistry, 1x 50 bp run.
~8 million reads/sample for each small-RNA sample and utilizing barcodes (48).
New Wildfire paired-end (wPE) 50 bp x 50 bp early access to start by the end of 2012.