Epigenetic Research and Analysis on the 5500xl Genetic Analyzer
The field of epigenetics examines the heritable genetic modifications not attributable to changes in primary DNA sequence. Epigenetic modifications have significant impacts on gene expression and therefore play a crucial role in the life of the cell. Lifestyle, nutrition, and environmental factors can all trigger epigenetic changes.
The most commonly studied epigenetic modifications are:
Expression of miRNAs can also have an impact on epigenetic mechanisms and modifications.
Unlike traditional microarrays, our ChIP-Seq technology offers you a way to study these interactions in an unbiased, hypothesis-free manner. Protein–DNA complexes are isolated by chromatin-immunoprecipitation and characterized by sequencing the associated DNA.
As CpG DNA methylation is not preserved during DNA amplification, studying methylation requires that the DNA be treated to either preserve or select for methylation status prior to DNA sequencing. Techniques used to prepare DNA for methylation analysis on the 5500xl Genetic Analyzer include: the selection of methylated DNA by affinity enrichment, enzymatic compartmentalization using methylation-sensitive enzymes, or chemical treatment of the DNA using bisulfite.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.