Whole-Genome Sequencing by Next-Generation (SOLiD®) Sequencing
Once the reference sequence for a given organism is completed, you can perform comparative sequencing to characterize the genetic diversity within the species or between closely related species. |
For Genetic Variations, the 5500 Genetic Analyzer is the Instrument of Choice
With superior throughput and accuracy, the 5500xl Genetic Analyzer is the platform of choice for cost-effective variant detection. Generating over 20 Gb of sequence a day with up to 99.99% accuracy gives you more mapped reads and less redundancy and “noise.” In addition, significantly higher physical coverage from mate-paired libraries compared to paired-end approaches enables you to get more accurate variant detection with much lower coverage than competitive platforms.
The Mate-Paired Library Advantage
- Mate-paired libraries enable you to detect structural variation such as insertions, deletions, inversions, translocations, duplications, and copy number variations in addition to SNPs.
- A broad variety of mate-paired insert sizes (0.6–6 Kb) provide you with the flexibility to accurately detect structural changes across a broad size range using 20 times less coverage than 500 bp paired-end reads.
- Preservation of strand orientation during analysis enables you to precisely pinpoint translocation breakpoints.
The 5500xl Genetic Analyzer delivers:
- Superior variant discovery—up to 99.99% accuracy with the Exact Call Chemistry (ECC) module allows detection of variants present in as little as 5% of your sample, with half the coverage needed for 99.0% accuracy
- Easy analysis—LifeScope™ analysis software gives you intuitive, fully annotated variant detection and reporting
- Flexible format—choose from fragment, paired-end, or mate-paired libraries of various sizes, and run them in whatever combination you like using the fully configurable 6-lane FlowChip
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Step-by-Step Guide to Whole Genome Resequencing
Application Notes
Publications
- The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse.
Publication: J Bacteriol. 2008 Apr;190(7):2597-606. Epub 2008 Feb 1.
Authors: Durfee, T., et al. - Whole-genome resequencing reveals loci under selection during chicken domestication.
Publication: Nature 464, 587-591 25 March 2010
Authors: Rubin C., et al. - Whole-genome sequencing in a patient with Charcot-Marie-Tooth neuropathy.
Publication: N Eng J Med 10 March 2010
Authors: Lupski J., et al. - Development of personalized tumor biomarkers using massively parallel sequencing.
Publication: Science Translational Medicine, 24 February 2010
Authors: Leary R., et al. - U87MG Decoded: The Genomic Sequence of a Cytogenetically Aberrant Human Cancer Cell Line.
Publication: PLoS Genetics, January 2010, volume 6, issue 1
Authors: Clark, et al. - Maternal Plasma DNA Analysis with Massively Parallel Sequencing by Ligation for Noninvasive; Prenatal Diagnosis of Trisomy 21
Publication: Clinical Chemistry 56:3 (2010)
Authors: Chiu, et al. - Complete Khoisan and Bantu genomes from southern Africa.
Publication: Nature, Vol 463, 18 February 2010
Authors: Schuster et al. - Whole-Exome Sequencing Identifies FAM20A Mutations as a Cause of Amelogenesis Imperfecta and Gingival Hyperplasia Syndrome
Publication: American Journal Human Genetics, (2011)
Authors: O'Sullivan J. et al. - Comparison of Sequence Reads Obtained from Three Next-Generation Sequencing Platforms
Publication: PLoS ONE (2011)
Authors: Suzuki S. et al. - Extensive genomic and transcriptional diversity identified through massively parallel DNA and RNA sequencing of eighteen Korean individuals
Publication: Nature Genetics (2011)
Authors: Ju. et al. - Global analysis of disease-related DNA sequence variation in 10 healthy individuals: Implications for whole genome-based clinical diagnostics
Publication: Genetics in Medicine (2011)
Authors: Moore. et al. - Demographic history and rare allele sharing among human populations
Publication: Gravel S et al (2011)
PNAS
