Exome Sequencing by Next-Generation (SOLiD®) Sequencing
Targeted resequencing is the comparative analysis of selected regions in a genome to identify genetic variants when compared to a reference. Increasingly targeted resequencing experiments are used in basic and clinical research as scientists look for causative variants for rare and complex diseases within populations.
The accuracy and throughput of the 5500xl Genetic Analyzer make it uniquely suited to targeted resequencing.
The TargetSeq™ Exome Enrichment System Provides Efficient Targeted Sequencing
- Targeted—focused, high-density probe design
- Economical—precapture sample multiplexing
- Platform-optimized—superior performance on 5500 Series Genetic Analyzers and SOLiD® Systems
- Integrated—streamlined data analysis with LifeScope™ Genomic Analysis Software
Exome sequencing is best performed using the TargetSeq™ Exome Enrichment System kits, which are based on an in-solution hybridization method for exome capture. The kits are optimally designed to seamlessly integrate into the 5500 Series Genetic Analysis Systems workflow.
Step-by-Step Guide to Targeted Resequencing Products
Decide on important experimental considerations: what type of variation you want to detect, in how many genes (or target regions), and how many samples?
- Targeted resequencing of candidate genes using selector probes.
Publication: Nucleic Acids Research (2010)
Authors: Johansson, et al.
- De novo mutations of SETBP1 cause Schinzel-Giedion syndrome.
Publication: Nature Genetics, May 2010
Authors: Hoischen, et al.
- PHF6 mutations in T-cell acute lymphoblastic leukemia.
Publication: Nature Genetics 42, 338-342 (2010)
Authors: Van Vlierberghe, et al.
- Accurate SNP and mutation detection by targeted custom microarray-based genomic enrichmentof short-fragment sequencing libraries.
Publication: Nucleic Acids Research, 2010, 1–9
Authors: Mokry, et al.
- Comparison of Three Targeted Enrichment Strategies on the SOLiD Sequencing Platform.
Publication: Plos One (2011)
Authors: Hedges D.J. et al.
- Population genetics in non- model organisms: II. Natural selection in marginal habitats revealed by deep sequencing on dual platforms.
Publication: Molecular Biology and Evolution (2011)
Authors: Zhou R. et al.
- De novo nonsense mutations in ASXL1 cause Bohring-Opitz syndrome.
Publication: Nature Genetics (2011)
Authors: Hoischen A. et al.
- Integrated genomic analyses of ovarian carcinoma.
Publication: Nature (2011)
Authors: Cancer Genome Atlas Research Network.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.