De Novo Sequencing
The initial generation of the primary genetic sequence of a particular organism is called de novo sequencing. A detailed genetic analysis of any organism is possible only after de novo sequencing has been performed.
De novo sequencing is typically accomplished by assembling individual sequence reads into longer contiguous sequences (contigs) or correctly ordered contigs (scaffolds) in the absence of a reference sequence.
Methods for De Novo Sequencing
Historically, de novo sequencing was carried out using capillary electrophoresis (CE) sequencers. With its long read lengths and high accuracy, CE-based sequencing made overlap consensus assembly the gold-standard technology for de novo projects. However, more recently, the high-throughput capabilities of massively parallel sequencing and the development of short-read assemblers have significantly reduced the time and cost associated with sequencing an entire genome.
De Novo Sequencing With the 5500xl Genetic Analyzer
De novo Sequencing Sample Prep for Next Generation Sequencing
De Novo Sequencing With the 3500 Genetic Analyzer
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.