DNA Sequencing

DNA Sequencing

DNA Sequencing is the process of reading nucleotide bases in a DNA molecule. Unlock the genome and answer biology’s most challenging questions with innovative and accessible sequencing solutions from Life Technologies.

For over 25 years, Life Technologies sequencers have contributed to significant scientific breakthroughs, including sequencing of the first human genome and the discovery of genes implicated in diseases like cystic fibrosis. Our ongoing innovation in sequencing technologies continues to drive new discoveries.

From whole genome sequencing to targeted sequencing of specific genomic regions, Life Technologies sequencing portfolio supports a wide range of throughput and research application needs for DNA sequencing.

Which platform is right for you?

Featured Sequencing Products

Semiconductor (Ion Torrent™) Next-Generation Sequencing	Sanger Sequencing	Next-Generation (SOLiD®) Sequencing

Whole Genome Sequencing
	Whole Genome Sequencing WGS entails sequencing the entire genome and comparing that to a reference genome in order to detect the full range of genetic variation such as SNPs, insertions, deletions, inversions, complex rearrangements, and copy number variation. Whole Genome Sequencing by Ion Semiconductor Sequencing Whole Genome Sequencing by Next-Generation Sequencing	De Novo Sequencing “From the beginning” or de novo sequencing is the method of building a reference genome. This is achieved by randomly fragmenting the target DNA, sequencing then assembling those individual fragments to build a draft or even finished genome. De Novo Sequencing by Ion Proton™ Semiconductor Sequencing
Targeted Sequencing
	Gene Sequencing Sequencing individual genes, gene regions, or sets of genes is a common targeted sequencing approach used in cancer and inherited disease research to screen known or discover novel germline and/or somatic mutations in focused areas of the genome. Amplicon Sequencing by Ion Semiconductor Sequencing Amplicon Sequencing by Sanger Sequencing	SNPs or Variants Validation Accurate variant confirmation using orthogonal technology (independent chemistry) follows discovery of novel variants. Life Technologies offers the gold standard and a fast approach for labs to validate their discoveries. SNP or variant validation by Ion Semiconductor Sequencing SNP Genotyping by Sanger Sequencing
	Exome Sequencing Targeted sequencing of the exome employs enrichment strategies that target coding exons, regulatory and 3’ untranslated regions, and other functionally annotated regions of interest to identify the functional variations (SNPs, indels) that are associated for a wide range of disorders and diseases. Exome Sequencing by Ion Semiconductor Sequencing Exome Sequencing by Next-Generation (SOLiD®) Sequencing	Mitochondrial Sequencing Variants in the mitochondrial genome are known to play a role in a number of human diseases. Targeted sequencing can be used to detect mutations present only in some copies of the mitochondrial genome (heteroplasmic mutations). Mitochondrial sequencing is also an important application for human identification and population genetics studies. Mitochondrial Sequencing by Ion Semiconductor Sequencing Mitochondrial Sequencing by Sanger Sequencing
	HLA Sequencing High resolution sequence-based HLA typing enables robust allele identification and comprehensive ambiguity resolution using Group Specific Sequencing Primers (GSSPs).	Microbial Identification Sequencing the 16S rRNA gene is an accurate and reliable method for microbial identification. It is used for environmental monitoring, contamination investigation, root cause analysis, raw materials testing, and food testing. Microbial Sequencing by Ion Semiconductor Sequencing Microbial Identification by Sanger Sequencing