Sanger Sequencing Solutions to Complement Your Next-Generation Sequencing Platform
As every researcher knows, no single application or technique tells the whole story. Often, it is the thoughtful combination of approaches that delivers the insight needed to move research forward. We have interviewed three researchers who are successfully employing various capillary electrophoresis (CE) applications to fill in some of the gaps or ambiguities in their next-generation sequencing (NGS) data. Read "Case studies in complementary capillary electrophoresis and next-generation applications" to find more about the utility of combining NGS and CE data to gain a more complete picture.  Sanger Sequencing for Mutation AnalysisSanger sequencing provides fast and accurate analysis of the mutations present at a frequency of 20% or greater in a sample. The combination of the new BigDye® Direct Cycle Sequencing Kit and a 3500 Series Genetic Analyzer provide the most comprehensive and reliable sequencing data.  Fragment Analysis for Somatic Mutation DetectionDetection of somatic mutations in samples like formalin-fixed, paraffin-embedded (FFPE) tissues may require higher levels of sensitivity and specificity than can be achieved with Sanger sequencing approaches. Fragment analysis provides solutions for achieving the high levels of sensitivity and specificity needed for somatic mutation detection. Sanger Sequencing for Genome FinishingSanger sequencing can perform in many situations where next-generation sequencing is limited, such as sequencing through difficult homopolymeric regions. Use the 3500 Genetic Analyzer for gap closure and to fill in regions of sequences that cannot be identified by next-generation sequencing approaches. Download "Resolving homopolymeric ambiguities using PCR-based fragment analysis” application note |
Review our comprehensive list of publications describing Sanger sequencing and fragment analysis applications used in conjunction with next-generation sequencing.
