Amplicon Sequencing by Sanger Sequencing
 Accurate heterozygous indel mutation detection on an Applied Biosystems Genetic Analyzer. Identifying heterozygous base positions or small insertions or deletions in genomic DNA is often employed to locate mutations or polymorphisms in diploid organisms, detect genetic rearrangements, and uncover rare variants. The combination of the new BigDye® Direct Cycle Sequencing Kit and the Applied Biosystems® 3500 Series Genetic Analyzers for comprehensive, reliable sequencing data on targeted DNA regions and in heterogeneous mixtures, is critical for countless life science and research applications. |
Step-by-Step Guide to Resequencing for Heterozygote Detection
 DNA extraction is a critical first step in the experimental workflow of DNA Sequencing and Fragment analysis. The overall quality, accuracy and length of the DNA sequence read can be significantly affected by characteristics of the sample itself, and the method chosen for nucleic acid extraction. Ideal methods will vary depending on the source or tissue type, how it was obtained from its source, and how the sample was handled or stored prior to extraction. Recommended Products: DNA Isolation |
Learn More
- DNA Resequencing Analysis of X-linked Mental Retardation (MRX) Variant
- Development of a Resequencing Workflow for Variant Analysis in the MLH1 and MSH2 Genes
- Development of a Workflow to Detect Sequence Variants in the BRCA1 and BRCA2 Genes
- A Workflow for Obtaining High Quality Sequencing Data from Bacterial Artificial Chromosome (BAC) DNA
Application Notes
Product Bulletin
Protocol Summary
Poster
Highlighted products for PCR Sequencing for Mutation Detection:
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For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
