Quantitative Fluorescence (qf-pcr)
  Enlarge Image Figure 1. Click to enlarge. Relative fluorescent quantitation (or quantitative fluorescence PCR (QF-PCR) is a technique used in a variety of fragment analysis applications that requires accurate peak height comparisons across multiple samples. Applications that utilize this technique include screening for loss of heterozygosity (LOH) using microsatellites or SNPs, aneuploidy assays, and detecting large chromosomal deletions. Figure 1: Detecting Loss of Heterozygosity (LOH) Electropherogram of a microsatellite marker in DNA from a healthy (top panel) and tumor sample (bottom panel) run on a Life Technologies 3130 Series Genetic Analyzer. The reduced peak height in the tumor sample indicates potential LOH in the sample. |
Step-by-Step Guide to Relative Fluorescent Quantification
 DNA extraction is a critical first step in the experimental workflow of DNA Sequencing and Fragment analysis. The overall quality, accuracy and length of the DNA sequence read can be significantly affected by characteristics of the sample itself, and the method chosen for nucleic acid extraction. Ideal methods will vary depending on the source or tissue type, how it was obtained from its source, and how the sample was handled or stored prior to extraction. Recommended Products: DNA Isolation |
Highlighted products for Fluorescent Quantification:
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