Microsatellite Instability (MSI)
Microsatellite instability (MSI) is a form of genomic instability due to reduced fidelity during the replication of repetitive DNA. Often occurring in tumor cells, MSI leads to the appearance of multiple alleles at microsatellite loci, which can be easily identified.
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Genomic Instability Identified by MSI Analysis
The electropherogram of the dinucleotide repeat marker D18S35 from a homozygous individual (top panel). The appearance of numerous extra alleles at lower molecular weights in the tumor sample (bottom panel) indicates significant genomic instability.
Step-by-Step Guide to Microsatellite Instability
DNA extraction is a critical first step in the experimental workflow of DNA Sequencing and Fragment analysis. The overall quality, accuracy and length of the DNA sequence read can be significantly affected by characteristics of the sample itself, and the method chosen for nucleic acid extraction. Ideal methods will vary depending on the source or tissue type, how it was obtained from its source, and how the sample was handled or stored prior to extraction.
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