Microsatellite Marker Analysis

Data from 18 Microsatellite Loci Co-Electrophoresed in a Single CapillaryEnlarge Image

Data from 18 Microsatellite Loci Co-Electrophoresed in a Single Capillary.
Life Technologies five-dye chemistry increases the number of microsatellite markers that can be run in a single capillary, maximizing throughput.

Microsatellite markers, or short tandem repeats (STR), are polymorphic DNA loci containing repeated nucleotide sequences, typically from 2 to 7 nucleotides per unit. The length of the repeated unit is the same for the majority of the repeats within an individual microsatellite locus; the number of repeats for a specific locus may differ, resulting in alleles of varying length.
The vast amount of data emerging for thousands of microsatellite markers across organisms makes microsatellite analysis a widely accepted tool for linkage and association studies, and also for identifying individual organisms (i.e., human identification or HID).

Microsatellite analysis includes PCR amplification of the microsatellite loci using fluorescently labeled primers; labeled PCR products are then analyzed by capillary electrophoresis (CE) or electrophoresis to separate the alleles by size.
Life Technologies offers a broad range of reagents, kits, instruments, and software that will help you achieve consistently precise and reliable results for a multitude of research applications.

New product bulletin for Microsatellite Applications

Applications

Human Identification

Microsatellite STR Analysis
Short tandem repeats (STRs), also called microsatellites, are polymorphic DNA loci present throughout the genome and used for linkage mapping studies, association studies, and identification of organisms.

Microsatellite Instability
Microsatellite instability (MSI) is a form of genomic instability arising from reduced fidelity during the replication of repetitive DNA that often occurs in tumor cells.

Microsatellite ISSR Analysis
Inter simple sequence repeat (ISSR) analysis is a technique commonly used for identifying individuals, commonly in plant species.

Regulatory status: For Research Use Only. Not for use in diagnostic procedures.

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