Methylation Sensitive Mobility Shift Assay
MSMSA is a DNA fragment analysis application used to assess the degree of methylation present in a given amplicon.
Samples from a fragment analysis assay can also be used in a downstream sequencing step, making this application ideal for screening. The assay primers are designed in regions that don’t contain CpGs using Methyl Primer Express® Software, so that PCR amplification is not dependent on methylation status.
Step-by-Step Guide to Methylation Sensitive Mobility Shift Assay
DNA extraction is a critical first step in the experimental workflow of DNA Sequencing and Fragment analysis. The overall quality, accuracy and length of the DNA sequence read can be significantly affected by characteristics of the sample itself, and the method chosen for nucleic acid extraction. Ideal methods will vary depending on the source or tissue type, how it was obtained from its source, and how the sample was handled or stored prior to extraction.
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